Nucleic acid fragments encoding isoflavone synthase
OwnerGary Fader, Woosuk Jung +3inventors
InventorsGary Fader, Woosuk Jung, Brian McGonigle, Joan Odell, Xiaodan Yu
Life of this patent
Estimating the term…
Abstract
This invention relates to an isolated nucleic acid sequence encoding isoflavone synthase. The invention also relates to the construction of chimeric sequences encoding all or a substantial portion of the enzymes, in sense or antisense orientation, wherein expression of the chimeric sequence results in production of altered levels of the enzyme in a transformed host cell.
Claims16
1-19. (canceled)
A plant comprising in its genome a chimeric polynucleotide comprising an isolated nucleic acid sequence encoding a polypeptide with isoflavone synthase activity having the amino acid sequence set forth in SEQ ID NO:66 wherein said chimeric polynucleotide is operably linked to at least one regulatory sequence.
26-33. (canceled)
A method of producing a plant with increased isoflavonoid content comprising
- (a) transforming a plant cell with a first chimeric polynucleotide comprising an isolated nucleic acid sequence encoding a polypeptide with isoflavone synthase activity having the amino acid sequence set forth in SEQ ID NO:66;
- (b) optionally transforming the plant cell with a second chimeric sequence comprising a nucleic acid sequence encoding a polypeptide that regulates expression of at least one enzyme of the phenylpropanoid pathway;
- and
- (c) growing the transformed plant cell under conditions that promote the regeneration of a whole plant from the transformed cell
- wherein the transformed plant regenerated from the transformed cell produces an amount of an isoflavonoid that is greater than the amount of the isoflavonoid that is produced in a plant that is regenerated from a plant cell that is not transformed with the chimeric polynucleotide of part (a).
41-50. (canceled)
Dependencies are inferred from the claim text.