Chemically-inducible promotors for the expression of proteins in plants
OwnerArtur Pfitzner, Bernhard Rothinventors
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Abstract
The present invention relates to nucleic acids containing selectively inducible regulatory sequences, particularly promoters, for a controlled expression of desired expression products in suitable host expression organisms such as transgenic plants. Further, the present invention relates to a method for detecting the activity of a regulatory sequence in suitable cells, wherein a transgene comprises a potential regulatory sequence operably linked to the Bax gene or a functional derivative thereof and the Bax expression correlates with the activity of the regulatory sequence in said cells.
Claims33
A recombinant nucleic acid containing at least a first nucleotide sequence operably linked to at least a second nucleotide sequence containing a transgene to be expressed, wherein the first nucleotide sequence contains a regulatory sequence selected from the group consisting of SEQ-ID-No. 1, SEQ-ID-No. 2, and a biologically active derivative thereof.
A method for detecting the activity of a regulatory sequence in suitable cells, comprising
- (a) preparing transformed cells, comprising at least a nucleotide sequence coding for the Bax gene or a biologically active derivative thereof, operably linked to a nucleotide sequence comprising a potential regulatory sequence,
- (b) treating the transformed cells with a chemical,
- (c) measuring the expression of the Bax gene or the biologically active derivative thereof in the transformed cells, and
- (d) correlating the Bax expression with the activity of the regulatory sequence.
(canceled)
Dependencies are inferred from the claim text.