US 2006/0241869 A1 · Computer systems and methods for inferring causality from cellullar constituent abundance data

Abstract

Methods for determining whether a molecule affects a disorder are provided. A cell from an organism is contacted with the molecule, or the molecule is expressed within the cell. A determination is made as to whether the RNA or protein expression in the cell of at least one open reading frame is changed relative to the expression of the reading frame in the absence of the molecule. Each such open reading frame is regulated by a promoter native to SEQ ID NOS: 5-9, 11-12, 14, 16, 18, 20-21, 23, 25, 27, 29, 31, 33 or homologs of the foregoing. A determination is made as to whether the molecule affects the disorder when the RNA or protein expression of the at least one reading frame is changed. Alternatively, a determination is made that the molecule does not affect the disorder when the RNA or protein expression of the at least one reading frame is unchanged.

Claims23

Dependent claims

A method for determining whether a candidate molecule affects a body weight disorder associated with an organism, comprising:

(a) contacting a cell from said organism with, or recombinantly expressing within the cell from said organism, said candidate molecule;
(b) determining whether the RNA expression or protein expression in said cell of at least one open reading frame is changed in step (a) relative to the expression of said open reading frame in the absence of the candidate molecule, each said open reading frame being regulated by a promoter native to a nucleic acid sequence selected from the group consisting of SEQ ID NO:
5, SEQ ID NO:
6, SEQ ID NO:
7, SEQ ID NO:
8, SEQ ID NO:
9, SEQ ID NO:
11, SEQ ID NO:
12, SEQ ID NO:
14, SEQ ID NO:
16, SEQ ID NO:
18, SEQ ID NO:
20, SEQ ID NO:
21, SEQ ID NO:
23 and homologs of each of the foregoing;
and
(c) determining that the candidate molecule affects a body weight disorder associated with said organism when the RNA expression or protein expression of said at least one open reading frame is changed, or determining that the candidate molecule does not affect a body weight disorder associated with said organism when the RNA expression or protein expression of said at least one open reading frame is unchanged.

14 more elements

A method of treating or preventing a body weight disorder comprising administering to a subject in which treatment is desired a therapeutically effective amount of a compound that antagonizes in the subject a protein comprising a sequence selected from the group consisting of SEQ ID NO:

1, SEQ ID NO:
2, SEQ ID NO:
3, SEQ ID NO:
4, SEQ ID NO:
10, SEQ ID NO:
13, SEQ ID NO:
15, SEQ ID NO:
17, SEQ ID NO:
19, SEQ ID NO:
22, SEQ ID NO:
24 and homologs of each of the foregoing.

8 more elements

Dependencies are inferred from the claim text.

Computer systems and methods for inferring causality from cellullar constituent abundance data

Life of this patent

Computer systems and methods for inferring causality from cellullar constituent abundance data

Life of this patent

Abstract

Claims23