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US 2006/0240502 A1Published application
GTP cyclohydrolase II as a target for fungicides
AssigneeBASF SE
InventorsAnnette Freund, Franz Rohl, Henning Althofer, Marvin Karos, Bruno Kaesler, Thierry Lacour
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Abstract
The present invention relates to the identification of fungal GTP cyclohydrolase II as a target for fungicides, to a method for identifying antifungal agents based on fungal GTP cyclohydrolase II, and also to the use of compounds identified as fungicides via the abovementioned method.
Claims8
1-21. (canceled)
A method for determination of GTP cyclohydrolase I or II activity comprising the steps of:
- a) adding GTP or GTP analog, NAD+ and formate dehydrogenase to a sample comprising GTP cyclohydrolase II or I;
- and
- b) determining a NADH content of the sample.
A method for identification of inhibitors of GTP cyclohydrolase I or II comprising the steps of:
- a) adding GTP or GTP analog, NAD+ and formate dehydrogenase to a first sample comprising GTP cyclohydrolase I or II;
- b) adding formate, NAD+ and formate dehydrogenase to a second sample comprising GTP cyclohydrolase I or II;
- c) adding to the first sample and to the second sample, a candidate compound;
- d) determining the activity of the first sample and the second sample;
- e) selecting candidate compounds that show inhibition in the presence of GTP and no inhibition in the presence of formate.
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Dependencies are inferred from the claim text.