Stress-related polypeptides and uses therefor
OwnerBret Cooperinventor
Life of this patent
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Abstract
Disclosed are proteins, and nucleic acids encoding such proteins, involved in or associated with the stress response (both biotic and abiotic stress) in plants. Also disclosed are uses for such proteins.
Claims46
An isolated nucleic acid molecule encoding a stress-related polypeptide, wherein the polypeptide binds in a yeast two hybrid assay to a fragment of a protein selected from the group consisting of OsGF14-c (SEQ ID NO:
- 113), OsDAD1 (SEQ ID NO:
- 128), Os006819-2510 (SEQ ID NO:
- 20), OsCRTC (SEQ ID NO:
- 134), OsSGT1 (SEQ ID NO:
- 144), OsERP (SEQ ID NO:
- 146), OsCHIB1 (SEQ ID NO:
- 152), OsCS (SEQ ID NO:
- 156), OsPP2A-2 (SEQ ID NO:
- 164), and OsCAA90866 (SEQ ID NO:
- 170).
An isolated nucleic acid molecule encoding a stress-related polypeptide, wherein the nucleic acid molecule is selected from the group consisting of:
- (a) a nucleic acid molecule encoding a polypeptide comprising an amino acid sequence of one of even numbered SEQ ID NOs:
- 2-112;
- (b) a nucleic acid molecule comprising a nucleic acid sequence of one of odd numbered SEQ ID NOs:
- 1-111;
- (c) a nucleic acid molecule that has a nucleic acid sequence at least 90% identical to the nucleic acid sequence of the nucleic acid molecule of (a) or (b);
- (d) a nucleic acid molecule that hybridizes to (a) or (b) under conditions of hybridization selected from the group consisting of:
- (i) 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO4, 1 mM ethylenediamine tetraacetic acid (EDTA) at 50° C. with a final wash in 2× standard saline citrate (SSC), 0.1% SDS at 50° C.;
- (ii) 7% SDS, 0.5 M NaPO4, 1 mM EDTA at 50° C. with a final wash in 1×SSC, 0.1% SDS at 50° C.;
- (iii) 7% SDS, 0.5 M NaPO4, 1 mM EDTA at 50° C. with a final wash in 0.5×SSC, 0.1% SDS at 50° C.;
- (iv) 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO4, 1 mM EDTA at 50° C. with a final wash in 0.1×SSC, 0.1% SDS at 50° C.;
- and
- (v) 7% sodium dodecyl sulfate (SDS), 0.5 M NaPO4, 1 mM EDTA at 50° C. with a final wash in 0.1×SSC, 0.1% SDS at 65° C.;
- (e) a nucleic acid molecule comprising a nucleic acid sequence fully complementary to (a);
- and
- (f) a nucleic acid molecule comprising a nucleic acid sequence that is the full reverse complement of (a).
An isolated stress-related polypeptide, wherein the polypeptide binds in a yeast two hybrid assay to a fragment of a protein selected from the group consisting of OsGF14-c (SEQ ID NO:
- 113), OsDAD1 (SEQ ID NO:
- 128), Os006819-2510 (SEQ ID NO:
- 20), OsCRTC (SEQ ID NO:
- 134), OsSGT1 (SEQ ID NO:
- 144), OsERP (SEQ ID NO:
- 146), OsCHIB1 (SEQ ID NO:
- 152), OsCS (SEQ ID NO:
- 156), OsPP2A-2 (SEQ ID NO:
- 164), and OsCAA90866 (SEQ ID NO:
- 170).
A method for modulating stress response of a plant cell comprising introducing into the plant cell an expression cassette comprising an isolated nucleic acid molecule encoding a stress-related polypeptide, wherein the polypeptide binds in a yeast two hybrid assay to a fragment of a protein selected from the group consisting of OsGF14-c (SEQ ID NO:
- 113), OsDAD1 (SEQ ID NO:
- 128), Os006819-2510 (SEQ ID NO:
- 20), OsCRTC (SEQ ID NO:
- 134), OsSGT1 (SEQ ID NO:
- 144), OsERP (SEQ ID NO:
- 146), OsCHIB1 (SEQ ID NO:
- 152), OsCS (SEQ ID NO:
- 156), OsPP2A-2 (SEQ ID NO:
- 164), and OsCAA90866 (SEQ ID NO:
- 170).
Dependencies are inferred from the claim text.