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OwnerTeruo Okano, Kohji Nishida +1inventors
InventorsTeruo Okano, Kohji Nishida, Masayuki Yamato
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The present invention has as its objective providing acorneal epithelium forming cell sheet that will adhere well to an anterior segment tissue. To attain this objective, a corneal epithelium forming cell sheet is produced by a process comprising the steps of cultivating under specified conditions corneal epithelium forming cells on a cell culture support comprising a substrate having its surface covered with a temperature responsive polymer of which the hydrating force varies in a temperature range of 0-80° C., optionally stratifying the layer of cultured cells, and thereafter, (1) adjusting the temperature of the culture solution to either above an upper critical dissolution temperature or below a lower critical dissolution temperature, (2) bringing the cultured corneal epithelium forming cells into close contact with a carrier, and (3) detaching the sheet together with the carrier under specified conditions.
A corneal epithelium forming cell sheet in close contact with a carrier.
A process for producing a corneal epithelium forming cell sheet, comprising the steps of cultivating corneal epithelium forming cells on a cell culture support comprising a substrate having its surface covered with a temperature responsive polymer of which the hydrating force varies in a temperature range of 0-80° C. with respect to water, optionally stratifying the layer of cultured cells by the usual method, and thereafter,
Dependencies are inferred from the claim text.