Malignant Cells and Method for Selecting the Same
OwnerPranela Rameshwarinventor
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Abstract
The present invention is a method for selecting an enriched population of malignant cells. It has been found that by depleting fibroblasts from a population of cells obtained from a tissue sample; selecting epithelial cells from the fibroblast-depleted population; and culturing the selected epithelial cell population in the presence of bone marrow stromal cells, an enriched population of malignant cells can be selected. In particular, when the malignant cells are constructively passaged on the bone marrow stromal cells, anchorage-independent cells can be obtained. Advantageously, the instant method provides malignant cells which exhibit a preference for bone marrow stromal cells.
Claims4
A method for selecting an enriched population of malignant cells comprising
- depleting fibroblasts from a population of cells obtained from a tissue sample;
- selecting epithelial cells from the fibroblast-depleted population;
- and
- culturing the selected epithelial cell population in the presence of bone marrow stromal cells so that an enriched population of malignant cells is selected.
Dependencies are inferred from the claim text.