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US 2006/0234368 A1Published application
Alga-origin promoter, intron and terminator
AssigneeTakara Bio Inc.
InventorsSatoru Fukuda, Naotsune Saga, Toshiharu Ohba, Sawako Nagashima, Shuichi Takahashi, Kiyozo Asada, Ikunoshin Kato
Life of this patent
Estimating the term…
Abstract
A promoter, an intron and a terminator by which a useful foreign gene can be efficiently expressed in algae and which are thus useful in breeding algae using genetic engineering techniques.
Claims18
An isolated DNA that exhibits a promoter activity in an alga, which is selected from the group consisting of (a) to (c) below:
- (a) a DNA having a nucleotide sequence of SEQ ID NO:9 or a portion thereof;
- (b) a DNA that is capable of hybridizing with the DNA of (a) or a strand complementary thereto under stringent conditions;
- and
- (c) a DNA having a nucleotide sequence in which one or more nucleotide is substituted, deleted, inserted or added in the DNA of (a).
1 more element
An isolated DNA that exhibits an activity of increasing transcription efficiency of a gene in an alga, which is selected from the group consisting of (d) to (f) below:
- (d) a DNA having a nucleotide sequence of SEQ ID NO:10 or a portion thereof;
- (e) a DNA that is capable of hybridizing with the DNA of (d) or a strand complementary thereto under stringent conditions;
- and
- (f) a DNA having a nucleotide sequence in which one or more nucleotide is substituted, deleted, inserted or added in the DNA of (d).
1 more element
An isolated DNA that exhibits a promoter activity in an alga which has:
- a first isolated DNA that exhibits a promoter activity in an alga, which is selected from the group consisting of (a) to (c) below:
- (a) a DNA having a nucleotide sequence of SEQ ID NO:9 or a portion thereof;
- (b) a DNA that is capable of hybridizing with the DNA of (a) or a strand complementary thereto under stringent conditions;
- and
- (c) a DNA having a nucleotide sequence in which one or more nucleotide is substituted, deleted, inserted or added in the DNA of (a), and
- a second isolated DNA that exhibits an activity of increasing transcription efficiency of a gene in an alga, which is selected from the group consisting of (d) to (f) below, and which is located downstream of the first isolated DNA being adjacent thereto or separated by a DNA fragment of 1 to 10,000 nucleotides therefrom:
- (d) a DNA having a nucleotide sequence of SEQ ID NO:10 or a portion thereof;
- (e) a DNA that is capable of hybridizing with the DNA of (d) or a strand complementary thereto under stringent conditions;
- and
- (f) a DNA having a nucleotide sequence in which one or more nucleotide is substituted, deleted, inserted or added in the DNA of (d).
7 more elements
An isolated DNA that exhibits a terminator activity in an alga, which is selected from the group consisting of (g) to (i) below:
- (g) a DNA having a nucleotide sequence of SEQ ID NO:11 or a portion thereof;
- (h) a DNA that is capable of hybridizing with the DNA of (g) or a strand complementary thereto under stringent conditions;
- and
- (i) a DNA having a nucleotide sequence in which one or more nucleotide is substituted, deleted, inserted or added in the DNA of (g).
1 more element
A recombinant DNA in which an objective gene is linked, such that the gene can be expressed, to any one of (1) to (3) below:
- (1) an isolated DNA that exhibits a promoter activity in an alga, which is selected from the group consisting of (a) to (c) below:
- (a) a DNA having a nucleotide sequence of SEQ ID NO:9 or a portion thereof;
- (b) a DNA that is capable of hybridizing with the DNA of (a) or a strand complementary thereto under stringent conditions;
- and
- (c) a DNA having a nucleotide sequence in which one or more nucleotide is substituted, deleted, inserted or added in the DNA of (a);
- (2) an isolated DNA that exhibits an activity of increasing transcription efficiency of a gene in an alga, which is selected from the group consisting of (d) to (f) below:
- (d) a DNA having a nucleotide sequence of SEQ ID NO:10 or a portion thereof;
- (e) a DNA that is capable of hybridizing with the DNA of (d) or a strand complementary thereto under stringent conditions;
- and
- (f) a DNA having a nucleotide sequence in which one or more nucleotide is substituted, deleted, inserted or added in the DNA of (d);
- and
- (3) an isolated DNA that exhibits a terminator activity in an alga, which is selected from the group consisting of (g) to (i) below:
- (g) a DNA having a nucleotide sequence of SEQ ID NO:11 or a portion thereof;
- (h) a DNA that is capable of hybridizing with the DNA of (g) or a strand complementary thereto under stringent conditions;
- and
- (i) a DNA having a nucleotide sequence in which one or more nucleotide is substituted, deleted, inserted or added in the DNA of (g).
13 more elements
A vector containing any one of (1) to (3) below:
- (1) an isolated DNA that exhibits a promoter activity in an alga, which is selected from the group consisting of (a) to (c) below:
- (a) a DNA having a nucleotide sequence of SEQ ID NO:9 or a portion thereof;
- (b) a DNA that is capable of hybridizing with the DNA of (a) or a strand complementary thereto under stringent conditions;
- and
- (c) a DNA having a nucleotide sequence in which one or more nucleotide is substituted, deleted, inserted or added in the DNA of (a);
- (2) an isolated DNA that exhibits an activity of increasing transcription efficiency of a gene in an alga, which is selected from the group consisting of (d) to (f) below:
- (d) a DNA having a nucleotide sequence of SEQ ID NO:10 or a portion thereof;
- (e) a DNA that is capable of hybridizing with the DNA of (d) or a strand complementary thereto under stringent conditions;
- and
- (f) a DNA having a nucleotide sequence in which one or more nucleotide is substituted, deleted, inserted or added in the DNA of (d);
- and
- (3) an isolated DNA that exhibits a terminator activity in an alga, which is selected from the group consisting of (g) to (i) below:
- (g) a DNA having a nucleotide sequence of SEQ ID NO:11 or a portion thereof;
- (h) a DNA that is capable of hybridizing with the DNA of (g) or a strand complementary thereto under stringent conditions;
- and
- (i) a DNA having a nucleotide sequence in which one or more nucleotide is substituted, deleted, inserted or added in the DNA of (g).
13 more elements
An isolated DNA that encodes a polypeptide having an elongation factor activity, which is selected from the group consisting of (j) to (l) below:
- (j) a DNA having a nucleotide sequence of SEQ ID NO:1 or a portion thereof;
- (k) a DNA that is capable of hybridizing with the DNA of (j) or a strand complementary thereto under stringent conditions;
- and
- (l) a DNA having a nucleotide sequence in which one or more nucleotide is substituted, deleted, inserted or added in the DNA of (j).
1 more element
An isolated DNA that encodes a polypeptide having an elongation factor activity, which is selected from the group consisting of (m) to (o) below:
- (m) a DNA having a nucleotide sequence of SEQ ID NO:2 or a portion thereof;
- (n) a DNA that is capable of hybridizing with the DNA of (m) or a strand complementary thereto under stringent conditions;
- and
- (o) a DNA having a nucleotide sequence in which one or more nucleotide is substituted, deleted, inserted or added in the DNA of (m).
1 more element
A DNA encoding an amino acid sequence of SEQ ID NO:8.
Dependencies are inferred from the claim text.