Ascorbic acid production from D-glucose in yeast
OwnerPaola Branduardi, Michael Sauer +2inventors
InventorsPaola Branduardi, Michael Sauer, Diethard Mattanovich, Danilo Porro
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Abstract
Herein is disclosed a method of generating ascorbic acid from yeast transformed with a mannose epimerase. In a further embodiment, the yeast can be further transformed with a myoinositol phosphatase. In the method, the transformed yeast can produce L-ascorbic acid from D-glucose. The transformed yeast has been observed to have increased growth rate, cell density, or survival when cultured on appropriate media.
Claims25
A method of generating L-ascorbic acid, comprising:
- a) obtaining a recombinant yeast functionally transformed with a coding region encoding a mannose epimerase (ME);
- b) culturing the recombinant yeast in a medium comprising D-glucose, thereby forming L-ascorbic acid, and
- c) isolating the L-ascorbic acid.
A recombinant yeast, wherein the yeast is functionally transformed with a coding region encoding a mannose epimerase (ME).
A method of increasing the production, productivity, or yield of a product produced by a microorganism during fermentation, comprising:
- functionally transforming the microorganism with a coding region encoding a mannose epimerase (ME).
Dependencies are inferred from the claim text.