Method for the production or r-a-lipoic acid by fermentation
OwnerTobias Dasslerinventor
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Abstract
The invention relates to a method for the production of R-α-lipoic acid by fermentation, characterised in that a cell with a weakened lipoyl protein ligase A activity is cultivated in a culture medium, whereby the cell precipitates enantiomerically pure R-α-lipoic acid in the free form in the culture medium and the enantiomerically pure R-α-lipoic acid is separated off from the culture medium.
Claims12
A method for preparing enantiomerically pure R-α-lipoic acid, wherein a cell having an attenuated lipoyl protein ligase A activity is cultured in a culture medium, said cell secreting enantiomerically pure R-α-lipoic acid in free form into said culture medium and said enantiomerically pure R-α-lipoic acid being removed from said culture medium.
A cell secreting enantiomerically pure R-α-lipoic acid into a culture medium and having an attenuated lipoyl protein ligase A activity, wherein it has, instead of a wild-type lplA gene, an lplA allele which has, in the base pair range 367-465, a base substitution which results in the LplA protein activity being reduced by at least 50%, or having a deletion in the lplA gene.
Dependencies are inferred from the claim text.