# US 2006/0234358 A1 · Method for the microbial production of aromatic amino acids and other metabolites of the aromatic amino acid biosynthetic pathway > Agents: this data is available as JSON from the patentgrep API and MCP server — see https://patentgrep.com/llms.txt - Number: US 2006/0234358 A1 - Document ID: US20060234358A1 - Status: Published application - Kind: A1 - Application number: 10/513,424 - Filed: 2003-04-29 - Published: 2006-10-19 - Earliest claimed: 2002-05-02 - Inventors: Britta Anderlei; Georg Sprenger; Hermann Sahm; Johannes Bongaerts - Web page: https://patentgrep.com/patent/US20060234358A1 ## CPC - C12P 13/22: Tryptophan; Tyrosine; Phenylalanine; 3,4-Dihydroxyphenylalanine - C12Y 604/01001: Pyruvate carboxylase (6.4.1.1) - C12P: FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE {(brewing of beer C12C; producing vinegar C12J; producing specific peptides or proteins C07K; producing enzymes C12N9/00; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification C12N15/00; measuring or testing processes involving enzymes or microorganisms C12Q; measuring or testing processes involving nucleic acid amplification reactions C12Q1/6844; fermentation processes to form a food composition, A21 or A23; compounds in general, see the relevant compound class, e.g. C01, C07)} - C12Y: ENZYMES ## Abstract A process for the microbial production of aromatic amino acids and other metabolites of the aromatic amino acid biosynthetic pathway The invention relates to a process for the microbial production of aromatic amino acids and other metabolites of the aromatic amino acid biosynthetic pathway. Microbially produced substances such as fine chemicals, in particular aromatic amino acids or metabolites of the aromatics biosynthetic pathway, are of great economic interest, and the need for amino acids, for example, continues to increase. The inventors found that, after introducing a pyc gene sequence into microorganisms, it was possible to produce aromatic amino acids and also metabolites of the aromatic biosynthetic pathway in an improved manner. The process of the invention is particularly suitable for producing L-phenylalanine. ## Claims ### Claim 1 A process to improve the microbial production of aromatic amino acids and other metabolites of the aromatic amino acid biosynthetic pathway, which method comprises introducing a pyc gene sequence into a microorganism and allowing said production in said microorganism to occur, whereby said production is improved. ### Claim 2 (depends on 1) The process of claim 1, which further comprises amplifying the pyc gene sequence in said microorganism. ### Claim 3 (depends on 1) The process of claim 1, which further comprises increasing the copy number of the pyc gene sequence in said microorganism. ### Claim 4 (depends on 1) The process of claim 1, which further comprises increasing gene expression of the pvc gene sequence in said microorganism. ### Claim 5 (depends on 1) The process of claim 1, wherein said pyc gene sequence is derived from an organism from the group consisting of Corynebacteria, Rhizobia, Brevibacteria, Bacillus, Mycobacteria, Pseudomonas, Rhodopseudomonas, Campylobacter, Methanococcus and Saccharomyces. ### Claim 6 (depends on 5) The process of claim 5, wherein said pyc gene sequence is derived from Corynebacterium glutamicum. ### Claim 7 (canceled) ### Claim 8 (depends on 1) The process of claim 1, wherein said production is of L-phenylalanine, L-tryptophan and/or L-tyrosine. ### Claim 9 (depends on 1) The process of claim 1, wherein said microorganism is selected from the group consisting of Enterobacteriaceae, Serratia, Bacillus, Corynebacterium and Brevibacterium. ### Claim 10 (depends on 1) The process of claim 1, wherein said microorganism is Escherichia coli. ### Claim 11 (depends on 1) The process of claim 1, which further comprises fermenting the microorganism in a medium containing components selected from the group consisting of biotin, IPTG and essential growth substances. ### Claim 12 (canceled) ### Claim 13 (depends on 1) The process of claim 1, which further comprises inactivating or switching off at least one PEP-consuming enzyme in said microorganism. ### Claim 14 (depends on 13) The process of claim 13, which further comprises inactivating or switching off at least one enzyme selected from the group consisting of PEP carboxylases, PEP-dependent sugar phosphotranferases (PTS) and pyruvate kinases in said microorganism. ### Claim 15 (depends on 1) The process of claim 1, which process further comprises introducing a PEP-independent transport system for glucose uptake into said microorganism. ### Claim 16 (depends on 1) The process of claim 1, which process further comprises introducing a glucose-facilitator protein (Glf) into said microorganism. ### Claim 17 (depends on 1) The process of claim 1, which process further comprises introducing a glucose-facilitator protein from Zymomonas mobilis into a microorganism. ### Claim 18 (depends on 1) The process of claim 1 which process further comprises introducing sugar transport genes into said microorganism. ### Claim 19 (depends on 1) The process of claim 1, which process further comprises introducing a transaldolase and/or transketolase into said microorganism. ### Claim 20 (depends on 1) The process of claim 1, which process further comprises introducing a transketolase A and/or transketolase B from E. coli into said microorganism. ### Claim 21 (depends on 1) The process of claim 1, which further comprises deregulating and/or amplifying at least one enzyme selected from the group consisting of DAHP synthase, shikimate kinase, chorismate mutase and prephenate dehydratase.