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AssigneeThe Kitasato Institute
InventorsSatoshi Omura, Haruo Ikeda, Yumiko Ogasawara
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In the present invention, a recombination of gene groups of nemadectin aglycon biosynthesis is performed for obtaining C-13 hydroxylnemadectin, to which sugar groups can be attached, and a production strain which produces C-13 hydroxylnemadectin is produced. Further, C-13 glycosylnemadectin producing strain is prepared by introducing aveBI-BVIII genes involving glycosidation of avermectin and biosynthesis of oleandrose. As described, C-13 hydroxylnemadectin and C-13 glycosidated nemadectin can be obtained effectively by using the producing strain prepared by means of the molecular genetic technology, and improvement in the biological activity thereof can be expected.
A microorganism strain belonging to Streptomyces cyaneogriseus subspecies noncyanogenus and having ability to produce C-13 glycosidated nemadectin.
A microorganism strain belonging to Streptomyces cyaneogriseus subspecies noncyanogenus and having ability to produce C-13 hydroxylnemadectin.
A process for manufacturing C-13 hydroxylnemadectin comprising culturing a microorganism belonging to Streptomyces cyaneogriseus subspecies noncyanogenus and having ability to produce C-13 hydroxylnemadectin in a medium, producing and accumulating C-13 hydroxylnemadectin in a culture medium, and collecting C-13 hydroxylnemadectin from the cultured mass.
A process for manufacturing C-13 glycosidated nemadectin comprising culturing a microorganism belonging to Streptomyces cyaneogriseus subspecies noncyanogenus and having ability to produce C-13 glycosidated nemadectin in a medium, producing and accumulating C-13 glycosidated nemadectin in a culture medium, and collecting C-13 glycosidated nemadectin from the cultured mass.
A microorganism belonging to Streptomyces cyaneogriseus subspecies noncyanogenus, maintaining gene groups of avermectin aglycon biosynthesis of Streptomyces avermitilis and having ability to produce C-13 hydroxylnemadectin.
A microorganism belonging to Streptomyces cyaneogriseus subspecies noncyanogenus, maintaining gene groups of avermectin aglycon biosynthesis of Streptomyces avermitilis and having ability to produce C-13 glycosidated nemadectin
A nemadectin non-producing microorganism strain belonging to Streptomyces cyaneogriseus subspecies noncyanogenus and inserting viomycin resistant gene in the region coding nemadectin aglycon biosynthesis genes nemA3-4 operon KS10 (KS10 insertion mutant).
A microorganism strain belonging to Streptomyces cyaneogriseus subspecies noncyanogenus, maintaining avermectin aglycon biosynthesis genes aveA3-4 of Streptomyces avermitilis in the KS10 insertion mutant, and having ability to form a hybrid PKS with NemA1-2 and AVES3-4.
A microorganism strain belonging to Streptomyces cyaneogriseus subspecies noncyanogenus and having ability to form a hybrid PKS with NemA1-2 and AVES3-4, wherein the microorganism strain maintains a regulator gene aveR of avermectin biosynthesis genes of Streptomyces avermitilis.
A microorganism strain belonging to Streptomyces cyaneogriseus subspecies noncyanogenus and having ability to form a hybrid PKS with NemA1-2 and AVES3-4, wherein the microorganism strain maintains a regulator gene aveR of avermectin biosynthesis genes and an avermectin glycosidation and an oleandrose biosynthesis genes aveBI-BVIII of Streptomyces avermitilis.
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