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US 2006/0234348 A1Published application
Method of production of RNA polymerase protein
OwnerJozef Bujarski, Rafal Wierzchoslawskiinventors
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Abstract
A method of forming a stable RNA polymerase enzyme by expressing protein 2a in cells, pelleting the cells expressing protein 2a, macerating the cells expressing protein 2a to obtain a cell lysate, and filtering the cell lysate through an affinity resin. A stable RNA polymerase enzyme for copying RNA in vitro for use is disclosed.
Claims12
A method of forming a stable RNA polymerase enzyme by:
- expressing protein 2a in cells;
- pelleting the cells expressing protein 2a;
- macerating the cells expressing protein 2a to obtain a cell lysate;
- and
- filtering the cell lysate through an affinity resin.
2 more elements
A method of specific gene silencing by expressing protein 2a together with a specific RNA primer, thereby inducing specific gene silencing by copying only the selected RNA primer sequence.
A stable RNA polymerase enzyme for copying RNA in vitro.
Dependencies are inferred from the claim text.