Expression of mammalian proteins in Pseudomonas fluorescens
AssigneePfenex Inc.
InventorsDiane Retallack, Charles Squires, David Watkins, Stacey Lee, Frank Gaertner, Robert Shutter
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Abstract
The invention is a process for improved production of a recombinant mammalian protein by expression in a Pseudomonad, particularly in a Pseudomonas fluorescens organism. The process improves production of mammalian proteins, particularly human or human-derived proteins, over known expression systems such as E. coli in comparable circumstances Processes for improved production of isolated mammalian, particularly human, proteins are provided.
Claims38
1-30. (canceled)
A method for producing an antibody comprising:
- transforming a Pseudomonas fluorescens host cell with a nucleic acid encoding an antibody;
- expressing the nucleic acid encoding an antibody in the host cell;
- isolating the antibody.
A method for producing an antibody or antibody fragment comprising:
- transforming a Pseudomonas fluorescens host cell with a nucleic acid encoding an antibody or antibody fragment comprising at least two separate translational units;
- culturing the host cell;
- expressing the nucleic acid encoding an antibody or antibody fragment in the host cell so that the translational units are expressed in sequential fashion;
- isolating the antibody.
A method of producing an antibody in a Pseudomonas fluorescens host cell comprising:
- transforming a Pseudomonas fluorescens host cell with a nucleic acid encoding an antibody comprising at least two expression vectors;
- expressing the nucleic acid encoding an antibody in the host cell;
- isolating the antibody.
Dependencies are inferred from the claim text.