Human DNA topoisomerase I alpha
AssigneeHuman Genome Sciences, Inc.
InventorsYing-Fei Wei, Mark Adams, Robert Fleischmann
Life of this patent
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Abstract
Disclosed is a human is a hTopI-α polypeptide and DNA (RNA) encoding such hTopI-α polypeptide. Also provided is a procedure for producing such polypeptide by recombinant techniques and antibodies and antagonists against such polypeptide. Also provided are methods of using the antibodies and antagonist inhibitors to inhibit the action of hTopI-α for therapeutic purposes such as an antitumor agent, to detect an autoimmune disease, or retroviral infections and to treat adenocarcinoma of the colon. Diagnostic methods for detecting mutations in the coding sequence and alterations in the concentration of the polypeptides in a sample derived from a host are also disclosed.
Claims20
A method of producing an antibody that specifically binds the polypeptide of SEQ ID NO:2 comprising:
- (a) introducing a polypeptide comprising at least 50 contiguous amino acids of SEQ ID NO:2 into an animal;
- and
- (b) recovering said antibody.
A method of producing an antibody that specifically binds the full-length polypeptide encoded by the cDNA in ATCC Deposit No. 75714 comprising:
- (a) introducing a polypeptide comprising at least 50 contiguous amino acids encoded by ATCC Deposit No. 75714 into an animal;
- and
- (b) recovering said antibody.
A method of producing an antibody that specifically binds the polypeptide of SEQ ID NO:2 comprising:
- (a) screening a single chain or Fab expression library to identify an antibody that specifically binds a polypeptide comprising at least 50 amino acids of SEQ ID NO:2;
- and
- (b) recovering said antibody from said library.
A method of producing an antibody that specifically binds the polypeptide encoded by the cDNA in ATCC Deposit No. 75714 comprising:
- (a) screening a single chain or Fab expression library to identify an antibody that binds a polypeptide comprising at least 50 amino acids of the polypeptide encoded by the cDNA in ATCC Deposit No. 75714;
- and
- (b) recovering said antibody from said library.
Dependencies are inferred from the claim text.