Human heavy chain antibody expression in flamentous fungi
AssigneeNovozymes A/S
InventorJesper Vind
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Abstract
The present invention relates to a method for producing a functional human immunoglobulin, wherein a human heavy chain immunoglobulin, devoid of any light chain, is expressed, comprising the steps of: a) transforming a filamentous host cell with a recombinant construct encoding a modified human heavy chain immunoglobulin, wherein the modifications comprise one or more mutations in the region of the heavy chain protein involved in contact with the light chain; b) culturing said filamentous host cell under conditions promoting expression of said modified human heavy chain immunoglobulin; and c) recovering said modified human heavy chain immunoglobulin.
Claims16
1-7. (canceled)
A method for producing a functional human immunoglobulin, wherein a human heavy chain immunoglobulin, devoid of any light chain, is expressed, comprising the steps of:
- a) transforming a filamentous host cell with a recombinant construct encoding a modified human heavy chain immunoglobulin, wherein the modifications comprise one or more mutations in the region of the heavy chain protein involved in contact with the light chain;
- b) culturing said filamentous host cell under conditions promoting expression of said modified human heavy chain immunoglobulin;
- and
- c) recovering said modified human heavy chain immunoglobulin.
Dependencies are inferred from the claim text.