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AssigneesRiken, Japan Science and Technology Agency
InventorsShigeyuki Yokoyama, Mikako Shirouzu, Ayako Sakamoto, Kensaku Sakamoto
Estimating the term…
A method of expressing a protein having an unnatural amino acid integrated thereinto characterized by comprising expressing (A) a mutant tyrosyl tRNA synthase which is a mutant of Escherichia coli-origin tyrosyl tRNA synthase and has an elevated specificity for a unnatural tyrosine derivative compared with the specificity for tyrosine, (B) a suppressor tRNA originating in an eubacterium belonging to the genus Bacillus, Mycoplasma or Staphylococcus which is capable of binding to the above tyrosine derivative in the presence of the above mutant TyrRS, and (C) a desired protein gene having a nonsense mutation at a desired site in animal cells to thereby incorporate the above tyrosine derivative into the nonsense mutation site in the above protein.
An expression method for non-naturally-occurring amino acid-containing protein comprising:
Animal cells containing:
DNA having a sequence selected from the group consisting of SEQ. ID NO. 1, SEQ. ID NO. 30, SEQ. ID NO. 31 and SEQ. ID NO. 32.
An expression vector capable of being expressed from a control sequence recognized in animal cells comprising a sequence selected from the group consisting of SEQ. ID NO. 1, SEQ. ID NO. 30, SEQ. ID NO. 31 and SEQ. ID NO. 32.
Dependencies are inferred from the claim text.