Isomaltose synthase-knockout microorganism belonging eumycota
AssigneeAmano Enzyme Inc.
InventorsKanako Suzuki, Norihiro Tsukagoshi
Life of this patent
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Abstract
It is intended to provide a host microorganism whereby the expression of a gene encoding a target protein can be effectively elevated in the case of transferring the gene. It is also intended to provide a transformant whereby a target protein can be produced at a high efficiency. It is further intended to provide a production process whereby a target protein can be produced at a high productivity. A microorganism which belongs to Eumycota and lacks the major isomaltose synthase. A gene encoding a target protein is transferred into the microorganism to give a transformant. Then the transformant is cultured under conditions allowing the expression of the transferred gene to thereby produce the protein.
Claims8
A microorganism which belongs to Eumycota and lacks a major isomaltose synthase gene.
Aspergillus nidulans which lacks an α-glucosidase B gene.
A transformant obtained by introducing a foreign gene whose expression is induced by isomaltose into a microorganism which belongs to Eumycota and lacks a major isomaltose synthase gene.
A transformant obtained by introducing a foreign gene whose expression is induced by isomaltose into Aspergillus nidulans which lacks an α-glucosidase B gene.
Dependencies are inferred from the claim text.