Expression system for actinomycete-origin cytochrome p-450 in escherichia coli
OwnerAkira Arisawa, Ayako Kumedainventors
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Abstract
This invention relates to a system for the expression of cytochrome P-450 gene in host Escherichia coli, and provides Escherichia coli which contains actinomycete ferredoxin gene and also ferredoxin gene and ferredoxin reductase gene which are xenogenic to Escherichia coli. Thus, this invention is useful for the promotion of effective single oxygen atom insertional reaction of a substrate organic compound.
Claims20
A system for the expression of actinomycete cytochrome P-450 genes in host Escherichia coli, wherein said Escherichia Coli supports a recombinant DNA molecule which comprises xenogenic microorganism-originated ferredoxin gene, ferredoxin reductase gene as well as said cytochrome P-450 gene, in operable state.
Dependencies are inferred from the claim text.