Hematological assay and kit
OwnerAndreas Calatzis, Marianne Wilmerinventors
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Abstract
The present invention is directed to a kit and a method for a fast and direct determination of the coagulation potential of a sample of blood or plasma utilising a thrombin substrate. The kit comprises at least one activator of the plasmatic substrate with a KM preferably coagulation system and a thrombin less than or equal to 200 μM in a relatively low concentration with respect to the sample whereby the substrate is wholly consumed within 5 to 600 seconds. Observations are made leading to a determination of the maximum rate of substrate consumption.
Claims45
A kit for assessing the coagulation potential of a sample of blood or plasma with the aid of apparatus for incrementally or continuously detecting the consumption of a thrombin substrate, comprising:
- a) at least one activator of the blood coagulation system, and b) a thrombin substrate, together with instructions or other means enabling:
- i) the use of a quantity of substrate sufficiently small to be completely consumed by a specified quantity of normal blood or plasma, and ii) calculation of at least one value indicative of the coagulation potential, said value being dependent on the velocity of consumption of the substrate, wherein the amount and kinetic properties of the substrate are selected such that said consumption takes place within a range from 5 to 600 seconds.
A method of assessing the coagulation potential of a sample of blood or plasma comprising:
- a) mixing a blood or plasma sample with at least one activator of the blood coagulation system and a thrombin substrate, and if necessary incubating the mixture, b) incrementally or continuously determining the release of the conversion product of the thrombin substrate, and c) calculating at least one value indicative of the coagulation potential, wherein:
- i.) the quantity of substrate is sufficiently small to be completely consumed by a specified quantity of normal blood or plasma, iv.) the amount and kinetic properties of the substrate are selected such that said consumption takes place within a range from 5 to 600 seconds, v.) the said value calculated is dependent on the velocity of consumption of the substrate.
A kit for assessing the coagulation potential of a sample of blood or plasma with the aid of apparatus for incrementally or continuously detecting the consumption of a thrombin substrate, comprising:
- a) at least one activator of the blood coagulation system, and
- b) a thrombin substrate having a KM 200 μM or less, preferably 100 μM or less, more preferably 50 μM or less.
Dependencies are inferred from the claim text.