Modulation of angiogenesis through targeting of arginyl transferase (ATE1)

A method of screening for a modulator of N-terminal arginylation activity, comprising:

• a) contacting at least one potential modulator of N-terminal arginylation activity with a first cell that expresses a first reporter protein, wherein the first reporter protein has an N-terminal aspartic acid, glutamic acid or cysteine residue and a half-life that is affected by arginylation of the N-terminal residue;
• b) measuring the level of the reporter protein expressed by the first cell and the level of reporter protein expressed by a control cell not exposed to the at least one potential modulator;
• c) comparing the level of the reporter protein expressed by the first cell with the level of reporter protein expressed by the control cell, wherein a difference in levels indicates of modulation of N-terminal arginylation activity, thereby screening for a modulator of N-terminal arginylation activity.