Protein scaffolds and uses thereof
AssigneeAvidia Research Institute
InventorsWillem Stemmer, Martin Vogt, Joost Kolkman, Josh Silverman, Candace Swimmer
Life of this patent
Estimating the term…
Abstract
Specific monomer domains and multimers comprising the monomer domains are provided. Methods, compositions, libraries and cells that express one or more library member, along with kits and integrated systems, are also included in the present invention.
Claims37
A method for identifying a monomer domain that binds to a target molecule, the method comprising,
- a) providing a library of non-naturally-occurring monomer domains, wherein the monomer domain is selected from the group consisting of a Ca-EGF monomer domain, a Notch/LNR monomer domain, a DSL monomer domain, an Anato monomer domain, and an integrin beta monomer domain,
- wherein the Ca-EGF monomer domain comprises the following sequence:
- (SEQ ID NO:2)
- DxdEC1xx(xx)xxxxC2x(xx)xxxxxC3xNxxGxfxC4x(xxx)xC5x
- xgxxxxxxx(xxxxx)xxxC6[[,]];
- wherein the Notch/LNR monomer domain, comprises the following sequence:
- (SEQ ID NO:3)
- C1xx(xx)xxxC2xxxxxnGxC3xxxC4nxxxC5xxDGxDC6d;
- wherein the DSL monomer domain comprises the following sequence:
- (SEO ID NO:4)
- C1xxxYygxxC2xxfC3xxxxdxxxhxxC4xxxGxxxC5xxGWxGxxC6;
- wherein the Anato monomer domain comprises the following sequence:
- (SEQ ID NO:5)
- C1C2xdgxxxxx(x)xxxxC3exrxxxxxx(xx)xxC4xxxfxxC5C6;
- wherein the integrin beta monomer domain comprises the following sequence:
- (SED ID NO:6)
- C1xxC2xxxxpxC3xwC4xxxxfxxx(gx)xxxxRC5dxxxxLxxxgC6;
- and
- wherein “x” is any amino acid;
- b) screening the library of monomer domains for affinity to a first target molecule;
- and
- c) identifying at least one monomer domain that binds to at least one target molecule.
A non-naturally occurring protein comprising a monomer domain that specifically binds to a target molecule
- wherein the target molecule is not bound by a naturally-occurring monomer domain at least 90% identical to the non-naturally occurring monomer domain,
- wherein the non-naturally occurring monomer domain is selected from the group consisting of a Ca-EGF monomer domain, a Notch/LNR monomer domain, a DSL monomer domain, an Anato monomer domain, and an integrin beta monomer domain.
A library of proteins comprising non-naturally-occurring monomer domains, wherein the monomer domain is selected from the group consisting of a Ca-EGF monomer domain, a Notch/LNR monomer domain, a DSL monomer domain, an Anato monomer domain, and an integrin beta monomer domain,
- wherein the Ca-EGF monomer domain comprises the following sequence:
- (SEQ ID NO:2)
- DxdEC1xx(xx)xxxxC2x(xx)xxxxxC3xNxxGxfxC4x(xxx)xC5x
- xgxxxxxxx(xxxxx)xxxC6[[,]]
- wherein the Notch/LNR monomer domain, comprises the following sequence:
- (SEQ ID NO:3)
- C1xx(xx)xxxC2xxxxxnGxC3xxxC4nxxxC5xxDGxDC6;
- wherein the DSL monomer domain comprises the following sequence:
- (SEQ ID NO:4)
- C1xxxYygxxC2xxfC3xxxxdxxxhxxC4xxxGxxxC5xxGWxGxxC6;
- wherein the Anato monomer domain comprises the following sequence:
- (SEQ ID NO:5)
- C1C2xdgxxxxx(x)xxxxC3exrxxxxxx(xx)xxC4xxxfxxC5C6;
- wherein the integrin beta monomer domain comprises the following sequence:
- (SEQ ID NO:6)
- C1xxC2xxxxpxC3xwC4xxxxfxxx(gx)xxxxRC5dxxxxLxxxgC6;
- and
- wherein “x” is any amino acid.
Dependencies are inferred from the claim text.