Methods of diagnosing and treating diabetes and insulin resistance
OwnerBernard Allan, Francine Gregoire +4inventors
InventorsBernard Allan, Francine Gregoire, Brian Lavan, Shonna Moodie, Steve Waters, Chi-Wai Wong
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Abstract
The present invention provides compositions and methods for diagnosing and treating diabetes and insulin resistance. In particular, the invention provides methods of identifying modulators of the polynucleotides or polypeptides of the invention and using those modulators to treat diabetes, as well as methods of diagnosing diabetes by measuring the levels of the polynucleotides or polypeptides of the invention in a patient.
Claims28
A method for identifying an agent for treating a diabetic or pre-diabetic individual, the method comprising the steps of:
- (i) contacting an agent to a mixture comprising a polypeptide encoded by a nucleic acid that hybridizes under stringent conditions to a nucleic acid encoding SEQ ID NO:2, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:16, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:28, SEQ ID NO:30, or SEQ ID NO:34;
- and
- (ii) selecting an agent that modulates the expression or activity of the polypeptide or that binds to the polypeptide, thereby identifying an agent for treating a diabetic or pre-diabetic individual.
A method of introducing an expression cassette into a cell, the method comprising,
- introducing into the cell an expression cassette comprising a promoter operably linked to a polynucleotide encoding a polypeptide, wherein the polynucleotide hybridizes under stringent conditions to a nucleic acid encoding SEQ ID NO:2, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:16, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:28, SEQ ID NO:30, or SEQ ID NO:34.
A method of diagnosing an individual who has Type 2 diabetes or is prediabetic, the method comprising,
- detecting in a sample from the individual the level of a polypeptide or the level of a polynucleotide encoding the polypeptide, wherein the polynucleotide hybridizes under stringent conditions to a nucleic acid encoding an amino acid sequence selected from the group consisting of SEQ ID NO:2, SEQ ID NO:6, SEQ ID NO:8, SEQ ID NO:10, SEQ ID NO:16, SEQ ID NO:20, SEQ ID NO:22, SEQ ID NO:28, SEQ ID NO:30, and SEQ ID NO:34;
- wherein a modulated level of the polypeptide or polynucleotide in the sample compared to a level of the polypeptide or polynucleotide in either a lean individual or a previous sample from the individual indicates that the individual is diabetic or prediabetic.
An isolated nucleic acid encoding a polypeptide comprising the amino acid sequence of SEQ ID NO:10 or SEQ ID NO:28.
Dependencies are inferred from the claim text.