Linear amplification of rna

A method for linear amplification of RNA, the method comprising the steps of:

• (a) adding a poly dT-high heel primer, obtained by binding poly dT to the 3′-terminal end of a high-heel primer, to sample RNA, and then allowing the mixture to react at a temperature of about 65-75° C. so as to anneal the poly dT-high heel primer with the poly-A portion of the sample RNA;
• (b) reacting the annealed sample with reverse transcriptase reactant so as to synthesize a cDNA, thus forming an RNA/cDNA hybrid;
• (c) reacting the formed RNA/cDNA hybrid with enzyme reactant including RNaseH, DNA polymerase and DNA ligase, so as to synthesize a double-stranded cDNA;
• and
• (d) adding a high-heel primer, dNTP and DNA polymerase to the double-stranded cDNA and subjecting the mixture to linear PCR amplification, in which annealing and extension are performed at about the same temperature in a range of 65-75° C.