Bioarray chip reaction apparatus and its manufacture
AssigneeAffymetrix, Inc.
InventorsDonald Besemer, Virginia Goss, James Winkler
Life of this patent
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Abstract
A body 300 having a cavity 310 for mounting a substrate 120 fabricated with probe sequences at known locations according to the methods disclosed in U.S. Pat. No. 5,143,854 and PCT WO 92/10092 or others, is provided. The cavity includes inlets 350 and 360 for introducing selected fluids into the cavity to contact the probes. Accordingly, a commercially feasible device for use in high throughput assay systems is provided.
Claims49
A method of making probe chips comprising the steps of:
- forming a plurality of probe arrays on a wafer;
- separating said wafer into a plurality of chips, each of said chips comprising at least one probe array thereon;
- and
- mating at least one of said chips to a package, said package comprising a reaction chamber, said reaction chamber comprising inlets for flowing fluid therein, said at least one probe array in fluid communication with said reaction chamber.
An array assay device, said device comprising:
- (a) a substrate receiving element for receiving a substrate having at least on array thereon, said substrate receiving element comprising a bottom surface;
- and (b) a compression element for urging said bottom surface in a direction towards a substrate when present in said substrate receiving element so as to hold said bottom surface in a fixed position relative to said substrate.
A method for performing an array assay, said method comprising:
- (a) providing an array assay device comprising:
- (i) a bottom surface, (ii) a substrate receiving element for receiving a substrate having at least on array thereon, and (iii) a compression element for urging said bottom surface in a direction towards a substrate when present in said substrate receiving element so as to hold said bottom surface in a fixed position relative to said substrate;
- (b) positioning a substrate comprising at least one array in said substrate receiving element;
- (c) urging said bottom surface in a direction towards said positioned substrate using said compression element, whereby said bottom surface is fixed relative to said substrate present in said receiving element;
- and (e) contacting a sample to said at least one array.
An apparatus for packaging a substrate, said apparatus comprising:
- a substrate having a first surface and a second surface, said first surface comprising a probe array and said second surface being an outer periphery of said first surface;
- a body having a mounting surface, an upper surface, and a cavity bounded by said mounting surface and said upper surface, said second surface being attached to said cavity and said first surface being within said cavity;
- and
- a cover attached to said mounting surface for defining an upper boundary to said cavity;
- wherein said cavity comprises a diffuser and a concentrator, said diffuser and said concentrator permitting laminar fluid flow through said cavity.
Dependencies are inferred from the claim text.