Methods of enhancing isolation of RNA from biological samples
AssigneeLumigen, Inc.
InventorHashem Akhavan-Tafti
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Abstract
A method of enhancing the isolation of ribonucleic acids from samples of biological or cellular material is disclosed which uses a solution of an Agent during binding of nucleic acids onto a solid phase binding material which, after elution, enhances the recovery of RNA. Washing the solid phase and eluting the nucleic acid produces RNA in enhanced yield and/or purity. The use of the new method allows RNA to be captured and released in a form suitable for downstream processing in under five minutes. Preferred Agents according to the invention comprise monomeric, oligomeric, dendrimeric and polymeric organic compounds having multiple positive charges.
Claims20
A method for isolating RNA from a biological sample comprising the steps of:
- (a) forming a mixture of the sample and a solution of an Agent for enhancing recovery of RNA;
- (b) contacting the mixture with a solid phase binding material;
- (c) separating the solid phase binding material from the solution;
- and
- (d) dissociating the RNA from the solid phase binding material.
Dependencies are inferred from the claim text.