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AssigneeVIRxSYS Corporation
InventorsMadaiah Puttaraju, Edward Otto, Mariano Garcia-Blanco, Gerard McGarrity, Gary Temple, Lloyd Mitchell, Colette Cote, S. Mansfield
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The present invention provides methods and compositions for generating novel nucleic acid molecules through targeted spliceosomal mediated RNA trans-splicing. The compositions of the invention include pre-trans-splicing molecules (PTMs) designed to interact with a SERPINA1 target precursor messenger RNA molecule (target pre-mRNA) and mediate a trans-splicing reaction resulting in the generation of a novel chimeric RNA molecule (chimeric RNA). In particular, the PTMs of the present invention include those genetically engineered to interact with SERPINA1 target pre-mRNA so as to result in correction of SERPINA1 genetic defects responsible for AAT deficiency. The PTMs of the invention may also comprise sequences that are processed out of the PTM to yield duplex siRNA molecules directed specifically to mutant SERPIN A1 mRNAs. Such duplexed siRNAs are designed to reduce the accumulation of toxic AAT protein in liver cells. The methods and compositions of the present invention can be used in gene therapy for correction of SERPINA1 disorders such as AAT deficiency.
A cell comprising a nucleic acid molecule wherein said nucleic acid molecule comprises:
A cell comprising a nucleic acid molecule wherein said nucleic acid molecule comprises:
A cell comprising a nucleic acid molecule wherein said nucleic acid molecule comprises:
A cell comprising a recombinant vector wherein said vector expresses a nucleic acid molecule comprising:
A cell comprising a recombinant vector wherein said vector expresses a nucleic acid molecule comprising:
A cell comprising a recombinant vector wherein said vector expresses a nucleic acid molecule comprising:
A method of producing a chimeric RNA molecule in a cell comprising:
A method of producing a chimeric RNA molecule in a cell comprising:
A method of producing a chimeric RNA molecule in a cell comprising:
A nucleic acid molecule comprising:
A nucleic acid molecule comprising:
A nucleic acid molecule comprising:
A eukaryotic expression vector wherein said vector expresses a nucleic acid molecule comprising:
A eukaryotic expression vector wherein said vector expresses a nucleic acid molecule comprising:
A eukaryotic expression vector wherein said vector expresses a nucleic acid molecule comprising:
A method for correcting a SERPINA1 genetic defect in a subject comprising administering to said subject a nucleic acid molecule comprising:
A cell comprising a nucleic acid molecule wherein said nucleic acid molecule comprises:
A cell comprising a recombinant vector wherein said vector expresses a nucleic acid molecule comprising:
A method of producing a chimeric RNA molecule in a cell comprising:
A nucleic acid molecule comprising:
A eukaryotic expression vector wherein said vector expresses a nucleic acid molecule comprising:
The vector of claim 48 wherein the nucleic acid molecule further comprises a nucleotide sequence capable encoding a siRNA capable of binding to a mutant SERPINA1 transcript.
A method for correcting a SERPINA1 genetic defect in a subject comprising administering to said subject a nucleic acid molecule comprising:
Dependencies are inferred from the claim text.