Method and device for pcr-amplification and detection of nucleotide sequences
AssigneeSiemens Aktiengesellschaft
InventorsWalter Gumbrecht, Manfred Stanzel
Life of this patent
Estimating the term…
Abstract
A DNA-Chip includes a flat carrier and an array of spots containing probe molecules (oligonucleotides) which are arranged on said carrier. Each spot is associated with a microelectrode arrangement for impedance spectroscopic detection of binding events occurring between the probe molecules and target molecules (DNA fragments) applied by way of an analyte solution. In order to increase the sensitivity or the binding specific measuring effects of the biochip, the electrode arrangement is at least partially embedded in a hydrophilic reaction layer containing probe molecules and which is permeable to target molecules.
Claims19
A method for PCR amplification and detection of nucleotide sequences, comprising:
- using an array of a plurality of microspots forming analytical positions, said microspots including as probe molecule at least one immobilized oligonucleotide which is hybridizable with a target sequence to be identified of a DNA fragment;
- applying an analyte solution including PCR reagents and a plurality of target sequences to the microspots in such a way that it completely covers the array;
- subjecting the array to a thermocycling process to amplify the target sequences;
- and
- detecting hybridization events on probe molecules immobilized at one analytical position with the aid of a microelectrode arrangement.
Dependencies are inferred from the claim text.