Method and nucleic acids for the analysis of colon cell proliferative disorders
OwnerPeter Adorjan, Matthias Burger +4inventors
InventorsPeter Adorjan, Matthias Burger, Sabine Maier, Ralf Lesche, Susan Cottrell, Suzanne Mooney
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Abstract
The invention provides methods and nucleic acids for detecting, differentiating or distinguishing between colon cell proliferative disorders by analysis of one or more of the genes Versican, TPEF, H-Cadherin, Calcitonin, and EYA4. The invention further provides novel nucleic acid sequences useful for the cell proliferative disorder specific analysis of said genes as well as methods, assays and kits thereof.
Claims61
A nucleic acid comprising a sequence at least 18 bases in length of a segment of the chemically pretreated genomic DNA according to one of the sequences taken from the group comprising SEQ ID NO 6 to SEQ ID NO 25 and sequences complementary thereto.
An oligomer, in particular an oligonucleotide or peptide nucleic acid (PNA)-oligomer, said oligomer comprising at least one base sequence having a length of at least 9 nucleotides which is complementary to, or hybridises under moderately stringent or stringent conditions to a pretreated genomic DNA according to one of the SEQ ID NO 6 to SEQ ID NO 25 or sequences complementary thereto.
A method for detecting, differentiating or distinguishing between colon cell proliferative disorders associated with at least one gene and/or their regulatory regions from the group comprising Versican, TPEF, H-Cadherin, Calcitonin and EYA4 in a subject, said method comprising contacting a target nucleic acid in a biological sample obtained from said subject with at least one reagent or a series of reagents, wherein said reagent or series of reagents, distinguishes between methylated and non methylated CpG dinucleotides within the target nucleic acid.
A kit useful for detecting, differentiating or distinguishing between colon cell proliferative disorders, comprising:
- i) a bisulfite reagent;
- and
- ii) at least one nucleic acid molecule or peptide nucleic acid molecule comprising, in each case a contiguous sequence at least 9 nucleotides in length that is complementary to, or hybridises under moderately stringent or stringent conditions to a sequence selected from the group consisting of SEQ ID NO 1 to SEQ ID NO 25, and complements thereof.
Dependencies are inferred from the claim text.