Peptides for detection of antibody to porcine reproductive respiratory syndrome virus
AssigneeIDEXX Laboratories, Inc.
InventorEugene Krah
Life of this patent
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Abstract
The invention provides compositions and methods for the detection and quantification of PRRSV antibodies and antibody fragments using polypeptides.
Claims32
A composition of matter comprising a purified polypeptide consisting essentially of SEQ ID NO:
- 1 or SEQ ID NO:
- 18.
A purified fusion polypeptide comprising a polypeptide consisting essentially of SEQ ID NO:1 or SEQ ID NO:18, and one or more polypeptides not contiguously associated with SEQ ID NO:1 or SEQ ID NO:18 in nature.
A method of detecting antibodies that specifically bind reproductive respiratory syndrome virus (PRRSV) or a PRRSV polypeptide comprising:
- (a) contacting a purified polypeptide consisting essentially of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:18, SEQ ID NO:13, or a combination thereof, with a test sample suspected of comprising antibodies specific for PRRSV, under conditions that allow polypeptide/antibody complexes to form;
- (b) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that antibodies specific for PRRSV are present in the test sample, and wherein the absence of polypeptide/antibody complexes is an indication that antibodies specific for PRSSV are not present in the test sample.
A method of detecting a PRRSV infection in a mammal comprising:
- (a) obtaining a biological sample from a mammal suspected of having a PRRSV infection;
- (b) contacting a purified polypeptide consisting essentially of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:18, or SEQ ID NO:13, or a combination thereof, with the biological sample under conditions that allow polypeptide/antibody complexes to form;
- (c) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that the mammal has a PRSSV infection and wherein the absence of polypeptide/antibody complexes is an indication that the mammal does not have a PRRSV infection.
An antibody that specifically binds to at least one epitope of a PRRSV polypeptide, wherein said polypeptide is SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:18, or SEQ ID NO:13.
A method of detecting a PRRSV polypeptide or PRRSV in a sample comprising:
- (a) contacting one or more antibodies that specifically bind to at least one epitope of an PRRSV polypeptide, wherein said polypeptide comprises SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:18, or SEQ ID NO:13 with the sample under conditions that allow polypeptide/antibody complexes to form;
- (b) detecting polypeptide/antibody complexes;
- wherein the detection of polypeptide/antibody complexes is an indication that PRSSV or a PRSSV polypeptide is present in the sample and the absence of polypeptide/antibody complexes is an indication that PRSSV or a PRRSV polypeptide is not present in the sample.
A method of decreasing the incidence of false positives in a diagnostic assay that detects PRRSV antibodies specific for PRRSV ORF 7 comprising:
- using a PRRSV ORF 7 polypeptide comprising about 19 to about 28 N-terminal amino acid deletions as an antibody capture antigen in the diagnostic assay.
Dependencies are inferred from the claim text.