Compositions, methods and kits for enhancing the immunogenicity of a bacterial vaccine vector
AssigneeThe Trustees of the University of Pennsylvania
InventorsYvonne Paterson, Christian Peters
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Abstract
The present invention comprises methods for enhancing the immunogenicity of a bacterial vaccine vector and an antigen by passaging the bacterial vaccine vector through an animal.
Claims30
A method of enhancing the immunogenicity of a bacterial vaccine vector, the method comprising:
- a) administering to an animal the bacterial vaccine vector;
- b) passaging the bacterial vaccine vector through the animal;
- c) harvesting the bacterial vaccine vector from the animal, and;
- d) repeating step a), step b), and step c) until a maximum bacterial load in an organ is reached, thereby enhancing the immunogenicity of the bacterial vaccine vector.
A bacterial vaccine vector having enhanced immunogenicity wherein the immunogenicity of the bacterial vaccine vector is enhanced by
- a) administering to an animal the bacterial vaccine vector;
- b) passaging the bacterial vaccine vector through the animal;
- c) harvesting the bacterial vaccine vector from the animal, and;
- d) repeating step a), step b), and step c) until a maximum bacterial load in an organ is reached.
A method of enhancing the immunogenicity of an antigen expressed from a bacterial vaccine vector, the method comprising:
- a) administering to an animal the bacterial vaccine vector;
- b) passaging the bacterial vaccine vector through the animal;
- c) harvesting the bacterial vaccine vector from the animal, and;
- d) repeating step a), step b), and step c) until a maximum bacterial load in an organ is reached, thereby enhancing the immunogenicity of the antigen expressed from a bacterial vaccine vector.
A kit comprising a bacterial vaccine vector having enhanced immunogenicity, wherein the kit comprises an applicator and an instructional material for use thereof.
Dependencies are inferred from the claim text.