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US 2006/0233824 A1Published application
Polypeptides and immunizing compositions containing gram positive polypeptides and methods of use
AssigneeEpitopix, LLC
InventorsDaryll Emery, Darren Straub, Laura Wonderling, Lisa Herron Olson
Life of this patent
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Abstract
The present invention provides isolated polypeptides isolatable from a Staphylococcus spp. Also provided by the present invention are compositions that include one or more of the polypeptides, and methods for making and methods for using the polypeptides.
Claims29
A composition comprising:
- two isolated polypeptides having molecular weights of 88 kDa, 55 kDa, 38 kDa, 37 kDa, 36 kDa, 35 kDa, 33 kDa, or a combination thereof, wherein molecular weight is determined by electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel, wherein the polypeptides are isolatable from a Staphylococcus aureus when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator, and wherein the composition protects an animal against challenge with S. aureus ATCC strain 19636.
A composition comprising:
- isolated polypeptides having molecular weights of 88 kDa, 55 kDa, 38 kDa, 37 kDa, 36 kDa, 35 kDa, and 33 kDa, wherein molecular weight is determined by electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel, wherein the polypeptides are isolatable from a Staphylococcus aureus, and wherein the composition protects an animal against challenge with S. aureus ATCC strain 19636.
A method for treating an infection in a subject comprising:
- administering an effective amount of a composition to a subject having or at risk of having an infection caused by a Staphylococcus spp., wherein the composition comprises:
- two isolated polypeptides having molecular weights of 88 kDa, 55 kDa, 38 kDa, 37 kDa, 36 kDa, 35 kDa, 33 kDa, or a combination thereof, wherein molecular weight is determined by electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel, wherein the polypeptides are isolatable from a S. aureus when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator, and wherein the composition protects an animal against challenge with S. aureus ATCC strain 19636.
A method for treating a symptom in a subject comprising:
- administering an effective amount of a composition to a subject having an infection caused by a Staphylococcus spp., wherein the composition comprises:
- two isolated polypeptides having molecular weights of 88 kDa, 55 kDa, 38 kDa, 37 kDa, 36 kDa, 35 kDa, 33 kDa, or a combination thereof, wherein molecular weight is determined by electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel, wherein the polypeptides are isolatable from a S. aureus when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator, and wherein the composition protects an animal against challenge with S. aureus ATCC strain 19636.
A method for treating an infection in a subject comprising:
- administering an effective amount of a composition to a subject having or at risk of having an infection caused by a Staphylococcus spp., wherein the composition comprises:
- antibody that specifically binds two isolated polypeptides having molecular weights of 88 kDa, 55 kDa, 38 kDa, 37 kDa, 36 kDa, 35 kDa, 33 kDa, or a combination thereof, wherein molecular weight is determined by electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel, wherein the polypeptides are isolatable from a Staphylococcus aureus when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator.
A method for treating a symptom in a subject comprising:
- administering an effective amount of a composition to a subject having an infection caused by a Staphylococcus spp., wherein the composition comprises:
- antibody that specifically binds two isolated polypeptides having molecular weights of 88 kDa, 55 kDa, 38 kDa, 37 kDa, 36 kDa, 35 kDa, 33 kDa, or a combination thereof, wherein molecular weight is determined by electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel, wherein the polypeptides are isolatable from a Staphylococcus aureus when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator.
A method for decreasing colonization in a subject comprising:
- administering an effective amount of a composition to a subject colonized by a Staphylococcus spp., wherein the composition comprises:
- two isolated polypeptides having molecular weights of 88 kDa, 55 kDa, 38 kDa, 37 kDa, 36 kDa, 35 kDa, 33 kDa, or a combination thereof, wherein molecular weight is determined by electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel, wherein the polypeptides are isolatable from a S. aureus when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator, and wherein the composition protects an animal against challenge with S. aureus ATCC strain 19636.
A method for decreasing colonization in a subject comprising:
- administering an effective amount of a composition to a subject colonized by a Staphylococcus spp., wherein the composition comprises:
- antibody that specifically binds two isolated polypeptides having molecular weights of 88 kDa, 55 kDa, 38 kDa, 37 kDa, 36 kDa, 35 kDa, 33 kDa, or a combination thereof, wherein molecular weight is determined by electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel, wherein the polypeptides are isolatable from a Staphylococcus aureus when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator.
A kit for detecting antibody that specifically binds a polypeptide, comprising in separate containers:
- an isolated polypeptide having a molecular weight of 88 kDa, 55 kDa, 38 kDa, 37 kDa, 36 kDa, 35 kDa, 33 kDa, or a combination thereof, wherein molecular weight is determined by electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel, and wherein the polypeptide is isolatable from a Staphylococcus aureus when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator;
- and
- a reagent that detects an antibody that specifically binds the polypeptide.
A composition comprising two isolated polypeptides having molecular weights selected from 88 kDa, 55 kDa, 38 kDa, 37 kDa, 36 kDa, 35 kDa, and 33 kDa, wherein molecuar weight is determined by electrophoresis on a sodium dodecyl sulfate-polyacrylamide gel,
- wherein the isolated polypeptide with a molecular weight of 88 kDa has a mass fingerprint of at least 80% similarity to a mass fingerprint of an 88 kDa polypeptide expressed by Staphylococcus aureus ATCC strain 19636, and wherein the polypeptide is isolatable from a Staphylococcus aureus when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator;
- wherein the isolated polypeptide with a molecular weight of 55 kDa has a mass fingerprint of at least 80% similarity to a mass fingerprint of a 55 kDa polypeptide expressed by Staphylococcus aureus ATCC strain 19636, and wherein the polypeptide is isolatable from a Staphylococcus aureus when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator;
- wherein the isolated polypeptide with a molecular weight of 38 kDa has a mass fingerprint of at least 80% similarity to a mass fingerprint of a 38 kDa polypeptide expressed by Staphylococcus aureus ATCC strain 19636, and wherein the polypeptide is isolatable from a Staphylococcus aureus when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator;
- wherein the isolated polypeptide with a molecular weight of 37 kDa has a mass fingerprint of at least 80% similarity to a mass fingerprint of a 37 kDa polypeptide expressed by Staphylococcus aureus ATCC strain 19636, and wherein the polypeptide is isolatable from a Staphylococcus aureus when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator;
- wherein the isolated polypeptide with a molecular weight of 36 kDa has a mass fingerprint of at least 80% similarity to a mass fingerprint of a 36 kDa polypeptide expressed by Staphylococcus aureus ATCC strain 19636, and wherein the polypeptide is isolatable from a Staphylococcus aureus when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator;
- wherein the isolated polypeptide with a molecular weight of 35 kDa has a mass fingerprint of at least 80% similarity to a mass fingerprint of a 35 kDa polypeptide expressed by Staphylococcus aureus ATCC strain 19636, and wherein the polypeptide is isolatable from a Staphylococcus aureus when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator;
- and
- wherein the isolated polypeptide with a molecular weight of 33 kDa has a mass fingerprint of at least 80% similarity to a mass fingerprint of a 33 kDa polypeptide expressed by Staphylococcus aureus ATCC strain 19636, and
- wherein the polypeptide is isolatable from a Staphylococcus aureus when incubated in media comprising an iron chelator and not isolatable when grown in the media without the iron chelator.
6 more elements
Dependencies are inferred from the claim text.