Focussed antibody technology
OwnerJames Burnie, Ruth Matthews +2inventors
InventorsJames Burnie, Ruth Matthews, Gordon Rigg, Peter Williamson
Life of this patent
Estimating the term…
Abstract
The present invention concerns methods for identifying candidate sequences for antibody specific against an antigen produced by a micro-organism during an infection or against a vaccine, methods of manufacture of medicaments, and methods of treatment of patients using same. Also provided is a method for determining the efficacy of a vaccine, together with methods of vaccinating a patient.
Claims26
1-25. (canceled)
A method for identifying candidate sequences of at least the CDR3 region of antibodies specific against at least one of the group consisting of:
- at least one antigen produced by a micro-organism during an infection, and a vaccine, the method comprising the steps of:
- (i) with B cells isolated from at least one patient who has been infected by said micro-organism or administered said vaccine, sequencing at least the CDR3 region of the VH and/or VL coding regions of said B cells;
- and
- (ii) correlating said sequenced regions of the VH and VL coding regions of said B cells to identify a set of candidate sequences for at least a CDR3 region of antibodies specific against said at least one antigen produced by said micro-organism or against said vaccine, each of said set of candidate CDR3 sequences or a sequence of having at least 60% homology therewith occurring in total at a frequency of at least 1 percent in the set of sequences determined at step (i).
A method for determining the efficacy of a vaccine, comprising the steps of:
- (i) with B cells isolated from at least one patient who has been administered said vaccine, sequencing at least the CDR3 region of the VH and/or VL coding regions of said B cells;
- and
- (ii) correlating said sequenced region of the VH and/or VL coding regions of said B cells to identify a set of candidate sequences for at least the CDR3 region of antibodies specific against said vaccine, each of said set of CDR3 candidate sequences or a sequence having at least 60% homology therewith occurring in total at a frequency of at least 1 percent in the set of sequences determined at step (i).
Dependencies are inferred from the claim text.