Ring finger family proteins and uses related thereto
AssigneeProteologics, Inc.
InventorsDanny Ben-Avraham, Tsvika Greener, Liora Yaar
Life of this patent
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Abstract
The application provides, among other things, specificity domains, and methods for identifying and using specificity domains.
Claims19
A RING finger E3 ligase selective inhibitor, wherein the RING finger E3 ligase selective inhibitor interacts with a specificity domain of a RING finger E3 ligase.
A method of identifying the specificity domain of a RING Finger E3 ligase comprising:
- (a) identifying the RING domain of the RING finger E3 ligase;
- and
- (b) determining the position of the conserved Cysteine residues,
- wherein the specificity domain is the domain that lies between the 6th and 7th conserved Cysteine residue.
An isolated or recombinant polypeptide selected from the group consisting of:
- (a) a polypeptide consisting essentially of a specificity domain;
- (b) a fusion protein comprising a specificity domain of a RING finger E3 ligase and a second domain;
- and
- (c) a chimeric RING Finger E3 ligase in which the specificity domain is replaced by the specificity domain of a second RING Finger E3 ligase.
A method of inhibiting interaction of a RING Finger E3 ligase and a RING Finger E3 ligase associated protein, comprising administering to a subject in need thereof an agent that interacts with the specificity domain of the RING finger E3 ligase.
A method of identifying an inhibitor of a RING finger E3 ligase, comprising:
- (a) identifying the specificity domain of the RING finger E3 ligase;
- and
- (b) identifying an agent that binds to at least a portion of the specificity domain, wherein the agent identified in (b) is an inhibitor of the RING finger E3 ligase.
Dependencies are inferred from the claim text.