Established cell line of microglia
OwnerMakoto Sawadainventor
Life of this patent
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Abstract
A subcultivatable, established microglia having the following properties. (a) Form: Both or either of a macrophage-like or spherical form in the presence of a granulocyte-macrophage colony stimulation factor and a branched form similar to branched microglia present in the brain in the absence of the factor. (b) Functional characteristics: specific affinity for the brain highly poor phagocytic action. (c) Cell proliferation: proliferative depending upon a granulocyte-macrophage colony stimulation factor. Preparation, separation, and screening methods of the microglia, a pharmaceutical composition using the microglia, and a method for treatment of cerebral diseases using the composition.
Claims8
1-16. (canceled)
A method for the production of established microglia cell lines having specific affinity for the brain, which comprises the steps of:
- a) separating and purifying type-II microglia cells from microglia cells isolated from animals, and
- b) incubating the type II microglia cells with cytokines.
Dependencies are inferred from the claim text.