Recombinant adenoviral vectors and applications thereof
AssigneesInstitut National de la Recherche Agronomique (INRA), Ecole Nationale Veterinaire D'Alfort
InventorsMarc Eloit, Bernard Klonjkowski
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Abstract
The invention relates to novel recombinant adenoviruses which can be obtained from a replicating adenovirus by deleting all or part of the region of the genome of said replicating adenovirus corresponding to that located in the genome of canine adenovirus type 2 (GenBank J04368) between positions 311 and 499, the aforementioned deletion comprising all or part of the region of the genome of the original replicating adenovirus corresponding to that located between positions 311 and 401 in the genome of canine adenovirus type 2. The invention also relates to the uses of said adenoviruses, particularly for therapeutic purposes.
Claims21
1) A recombinant adenovirus which can be obtained from a replicating adenovirus by deleting all or part of the region of the genome of said replicating adenovirus corresponding to that located between positions 311 and 499 in the genome of type 2 canine adenovirus (GenBank J04368), with said deletion comprising all or part of the region of the genome of the original replicating adenovirus corresponding to that located between positions 311 and 401 in the genome of type 2 canine adenovirus.
16) A method for preparing a recombinant adenovirus by means of intermolecular homologous recombination in a prokaryotic cell, characterized in that it comprises the following steps:
- α) introducing, into said prokaryotic cell:
- (i) a plasmid comprising the genome of an adenovirus and a first selection gene;
- and (ii) a previously linearized DNA fragment which comprises a heterologous sequence flanked by sequences which are homologous to those flanking the site of said plasmid where the insertion is to be effected and which includes a second selection gene which differs from the first;
- and
- β) culturing said prokaryotic cell under selective conditions in order to make it possible to generate and select cells which harbor recombinant plasmids which are expressing the first and second selection genes, and
- γ) isolating the genome of said recombinant adenovirus from selected prokaryotic cells.
Dependencies are inferred from the claim text.