Liquid chromatograph control apparatus, method for perfoming liquid chromatography and computer program for controlling a liquid chromatograph

A computer program for controlling a liquid chromatograph, wherein the program causes a computer to function as:

• measured value storage that stores a measured retardation factor (Rf) value of a sample, which is obtained when components of the sample are separated on thin layer chromatography using eluent containing a plurality of ingredients at a specified mixture ratio, in association with the specified mixture ratio,
• rate-of-change-in-Rf-value storage that stores a rate of change in the retardation factor (Rf) value of the sample with respect to variation in mixture ratio of the ingredients of the eluent,
• mixture ratio calculator that determines a mixture ratio of the eluent at which a specified retardation factor value (RfO) of the sample is obtained, based on the measured retardation factor (Rf) value corresponding to the specified mixture ratio stored in the measured value storage and on the rate of change in the retardation factor (Rf) value stored in the rate-of-change-in Rf-value storage, and
• mixture ratio controller that outputs a control signal to control a mixture ratio of eluent fed into a column so that the retardation factor (Rf) value of the sample can be equivalent to the specified retardation factor value (RfO), based on calculated results by the mixture ratio calculator.