Electrophoretic apparatus and electrophoretic method
AssigneeHitachi High-Technologies Corporation
InventorsRyoji Inaba, Tomoyuki Sakai, Motohiro Yamazaki, Takashi Gomi, Kazumichi Imai
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Abstract
The present invention relates to detection of an emission spectrum by irradiating excitation light onto a plurality of electrophoretic paths and dispersing fluorescent light output from the electrophoretic paths in a direction approximately vertical to an electrophoretic direction. According to the invention, since an emission spectrum to be detected does not substantially change over time, it becomes possible to make observed emission spectrums completely correspond to various fluorescent dyes or various bases.
Claims15
An electrophoretic apparatus comprising:
- a plurality of electrophoretic paths;
- an irradiation unit that irradiates excitation light onto the electrophoretic paths arranged at predetermined intervals therebetween;
- a wavelength dispersion unit that disperses fluorescent light output from the electrophoretic paths according to the wavelength;
- and
- an optical detection unit that detects light from the wavelength dispersion unit so as to obtain an emission spectrum,
- wherein a wavelength dispersion direction of the wavelength dispersion unit is approximately perpendicular to an electrophoretic direction in each of the electrophoretic paths.
An electrophoretic apparatus comprising:
- a plurality of electrophoretic paths;
- an irradiation unit that irradiates excitation light onto the electrophoretic paths arranged at predetermined intervals therebetween;
- a wavelength dispersion unit that disperses fluorescent light output from the electrophoretic paths according to the wavelength;
- and
- an optical detection unit that detects light from the wavelength dispersion unit so as to obtain an emission spectrum,
- wherein a wavelength dispersion direction of the wavelength dispersion unit is slightly inclined with respect to a direction perpendicular to an electrophoretic direction in each of the electrophoretic paths.
An electrophoretic method comprising:
- electrophoresing a plurality of fluorescently marked samples by using a plurality of electrophoretic paths;
- irradiating excitation light onto the plurality of electrophoretic paths arranged at predetermined intervals therebetween;
- dispersing fluorescent light output from the electrophoretic paths according to the wavelength, in a direction approximately perpendicular to an electrophoretic direction in each of the electrophoretic paths;
- and
- detecting the wavelength-dispersed light so as to obtain an emission spectrum.
An electrophoretic method comprising:
- electrophoresing a plurality of fluorescently marked samples by using a plurality of electrophoretic paths;
- irradiating excitation light onto the plurality of electrophoretic paths arranged at predetermined intervals therebetween;
- dispersing fluorescent light output from the electrophoretic paths according to the wavelength, in a direction slightly inclined with respect to a direction perpendicular to an electrophoretic direction in each of the electrophoretic paths;
- and
- detecting the wavelength-dispersed light so as to obtain an emission spectrum.
Dependencies are inferred from the claim text.