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US 2006/0225155 A1Published application
Method for the production of stably transformed, fertile gramineae employing agrobacterium-mediated transformation of isolated gramineae zygotes
AssigneeSunGene GmbH
InventorJochen Kumlehn
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Abstract
The present invention relates to methods for the incorporation of DNA into the genome of a Gramineae plant, preferably a wheat plant, by means of Agrobacterium-mediated transformation employing isolated zygotes as target material.
Claims12
A method for producing a transgenic Gramineae plant comprising the steps of:
- (a) isolating a zygote from a Gramineae plant to be transformed in a way that said isolated zygote becomes substantially free from its naturally surrounding tissue,
- (b) introducing a DNA composition comprising a genetic component into the genome of said Gramineae plant, wherein said introduction is mediated by Agrobacterium transformation into said isolated zygote;
- (c) regenerating Gramineae plants from said zygotes which have received said genetic component;
- and
- (d) identifying a fertile, transgenic Gramineae plant whose genome has been altered through the stable introduction of said genetic component.
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Dependencies are inferred from the claim text.