US 2006/0225151 A1 · Polynucleotides and methods for making plants resistant to fungal pathogens

Abstract

This invention relates to polynucleotide sequences encoding a gene that can confer resistance to the plant pathogen Colletotrichum, which causes anthracnose stalk rot, leaf blight and top dieback in corn and other cereals. It further relates to plants and seeds of plants carrying chimeric genes comprising said polynucleotide sequences, which enhance or confer resistance to the plant pathogen Colletotrichum, and processes of making said plants and seeds. The invention further presents sequences that can be used as molecular markers that in turn can be used to identify the region of interest in corn lines resulting from new crosses and to quickly and efficiently introgress the gene from corn lines carrying said gene into other corn lines that do not carry said gene, in order to make them resistant to Colletotrichum and resistant to stalk rot.

Claims25

Dependent claims

An isolated polynucleotide comprising a polynucleotide selected from the group consisting of:

(a) a nucleotide sequence encoding a polypeptide conferring or enhancing resistance to Colletotrichum, wherein the polypeptide has an amino acid sequence of at least 75% identity, when compared to SEQ ID NO:3 based on the Needleman-Wunsch alignment algorithm;
(b) a complement of the nucleotide sequence, wherein the complement and the nucleotide sequence consist of the same number of nucleotides and are 100% complementary;
and
(c) a nucleotide sequence encoding a polypeptide conferring or enhancing resistance to Colletotrichum, wherein the polypeptide has an amino acid sequence of at least 75% identity, when compared to the sequence of the plasmid deposited as Patent Deposit No. NRRL B-30895 based on the Needleman-Wunsch alignment algorithm.

1 more element

Dependencies are inferred from the claim text.

Facts

Application

11/397,153Filed 4 Apr 2006

Priority

4 Apr 2005

Published

5 Oct 2006

Term ends

Not estimatedThe source records don’t support an estimate.

Classification13 codes

C12Q 1/6895
For detection or identification of organisms: for plants, fungi or algae
A01H 1/045
Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection: using molecular markers
A01H 5/10
Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy: seeds
A01H 6/4684
Gramineae or Poaceae, e.g. ryegrass, rice, wheat or maize: zea mays [maize]

Polynucleotides and methods for making plants resistant to fungal pathogens

Life of this patent

Polynucleotides and methods for making plants resistant to fungal pathogens

Life of this patent

Abstract

Claims25