Nucleic acid molecules and other molecules associated with soybean cyst nematode resistance

A method of introgressing an allele into a soybean plant comprising

• (A) crossing at least one SCN resistant soybean plant with at least one SCN sensitive soybean plant in order to form a segregating population,
• (B) screening said segregating population with one or more nucleic acid markers to determine if one or more soybean plants from said segregating population contains an rhg1 SCN resistant allele, or an Rhg4 SCN resistant allele,
• wherein said rhg1 SCN resistant allele is an allele having a deletion of 19 nucleotides corresponding to SEQ ID NO:
• 2 and encompassing position 48881, and said Rhg4 SCN resistant allele is an allele having one or more polymorphisms at a position in SEQ ID NO:
• 4 selected from the group consisting of 111933, 112065, 112101, and 112461, and
• (C) selecting, if present, one or more soybean plants of said segregating population containing said deletion, or said one or more polymorphisms located at a position in SEQ ID NO:
• 4.

A method of introgressing an allele into a soybean plant comprising

• (A) crossing at least one SCN resistant soybean plant with at least one SCN sensitive soybean plant in order to form a segregating population,
• (B) screening said segregating population with one or more nucleic acid markers to determine if one or more soybean plants from said segregating population contains an rhg1 SCN resistant allele, or an Rhg4 SCN resistant allele,
• wherein said rhg1 SCN resistant allele is an allele having one or more first polymorphisms located at a position in SEQ ID NO:
• 2 selected from the group consisting of 45173, 45309, 47057, 47140, 47208, 47571, 47617, 47796, 47856, 47937, 48012, 48060, 48073, 48135, 48279, 48413, 48681, 48881, 49012, and 493161, and said Rhg4 SCN resistant allele is an allele having one or more second polymorphisms at a position in SEQ ID NO:
• 4 selected from the group consisting of 111933, 112065, 112101, and 112461, and
• (C) selecting, if present, one or more soybean plants of said segregating population containing said one or more first polymorphisms, or said one or more second polymorphisms.

A method of introgressing an allele into a soybean plant comprising

• (A) crossing at least one SCN resistant soybean plant with at least one SCN sensitive soybean plant in order to form a segregating population,
• (B) screening said segregating population with one or more nucleic acid markers to determine if one or more soybean plants from said segregating population contains an rhg1 SCN resistant allele, or an Rhg4 SCN resistant allele,
• wherein said rhg1 SCN resistant allele is an allele having one or more first polymorphisms in a protein coding region corresponding to nucleotides 45163 to 45314, 45450 to 45509, 46941 to 48763 or 48975 to 49573 of SEQ ID NO:
• 2, and said Rhg4 SCN resistant allele is an allele having one or more second polymorphisms in a protein coding region corresponding to nucleotides 111805 to 113968 or 114684 to 115204 of SEQ ID NO:
• 4, and
• (C) selecting, if present, one or more soybean plants of said segregating population containing said one or more first polymorphisms, or said one or more second polymorphisms.

A method of introgressing an allele into a soybean plant comprising

• (A) crossing at least one SCN resistant soybean plant with at least one SCN sensitive soybean plant in order to form a segregating population,
• (B) screening said segregating population with one or more nucleic acid markers to determine if one or more soybean plants from said segregating population contains an rhg1 SCN resistant allele or an Rhg4 SCN resistant allele,
• wherein said rhg1 SCN resistant allele is an allele having a deletion of 19 nucleotides corresponding to SEQ ID NO:
• 2 and encompassing position 48881, and said Rhg4 SCN resistant allele is an allele having one or more polymorphisms within SEQ ID NO:
• 4, and
• (C) selecting, if present, one or more soybean plants of said segregating population containing said deletion, or said one or more polymorphisms within SEQ ID NO:
• 4.

A method of introgressing an allele into a soybean plant comprising

• (A) crossing at least one SCN resistant soybean plant with at least one SCN sensitive soybean plant in order to form a segregating population,
• (B) screening said segregating population with one or more nucleic acid markers to determine if one or more soybean plants from said segregating population contains an rhg1 SCN resistant allele, or an Rhg4 SCN resistant allele,
• wherein said rhg1 SCN resistant allele is an allele having one or more first polymorphisms in a protein coding region corresponding to nucleotides 45163 to 49573 of SEQ ID NO:
• 2, and said Rhg4 SCN resistant allele is an allele having one or more second polymorphisms in a protein coding region corresponding to nucleotides 111805 to 115204 of SEQ ID NO:
• 4, and
• (C) selecting, if present, one or more soybean plants of said segregating population containing said one or more first polymorphisms, or said one or more second polymorphisms.

A transformed plant having a nucleic acid molecule which comprises:

• (A) an exogenous promoter region which functions in a plant cell to cause the production of a mRNA molecule;
• (B) a structural nucleic acid molecule encoding a protein or fragment thereof comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
• 1097, 1100, 1098, 1101, 1102-1115;
• and (C) a 3′ non-translated sequence that functions in the plant cell to cause termination of transcription and addition of polyadenylated ribonucleotides to a 3′ end of the mRNA molecule.

A transformed plant having a nucleic acid molecule which comprises:

• (A) an exogenous promoter region which functions in a plant cell to cause the production of a mRNA molecule;
• (B) a structural nucleic acid molecule encoding a protein or fragment thereof comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
• 1099, 1116-1119;
• and (C) a 3′ non-translated sequence that functions in the plant cell to cause termination of transcription and addition of polyadenylated ribonucleotides to a 3′ end of the mRNA molecule.

A transgenic seed having a nucleic acid molecule which comprises:

• (A) an exogenous promoter region which functions to cause the production of a mRNA molecule;
• (B) a structural nucleic acid molecule encoding a protein or fragment thereof comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
• 1097, 1100, 1098, 1101, 1102-1115;
• and (C) a 3′ non-translated sequence that functions to cause termination of transcription and addition of polyadenylated ribonucleotides to a 3′ end of the mRNA molecule.

A transgenic seed having a nucleic acid molecule which comprises:

• (A) an exogenous promoter region which functions to cause the production of a mRNA molecule;
• (B) a structural nucleic acid molecule encoding a protein or fragment thereof comprising an amino acid sequence selected from the group consisting of SEQ ID NOs:
• 1099, 1116-1119;
• and (C) a 3′ non-translated sequence that functions to cause termination of transcription and addition of polyadenylated ribonucleotides to a 3′ end of the mRNA molecule.

A transformed plant having a nucleic acid molecule which comprises:

• (A) an exogenous promoter region which functions in a plant cell to cause the production of a mRNA molecule;
• (B) a structural nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
• 5, 6, 8-23, 28-43, complements thereof, and fragments of either;
• and (C) a 3′ non-translated sequence that functions in the plant cell to cause termination of transcription and addition of polyadenylated ribonucleotides to a 3′ end of the mRNA molecule.

A transformed plant having a nucleic acid molecule which comprises:

• (A) an exogenous promoter region which functions in a plant cell to cause the production of a mRNA molecule;
• (B) a structural nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
• 7, 44-77 and 50-53, complements thereof, and fragments of either;
• and (C) a 3′ non-translated sequence that functions in the plant cell to cause termination of transcription and addition of polyadenylated ribonucleotides to a 3′ end of the mRNA molecule.

A transgenic seed having a nucleic acid molecule which comprises:

• (A) an exogenous promoter region which functions to cause the production of a mRNA molecule;
• (B) a structural nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
• 5, 6, 8-23, 28-43, complements thereof, and fragments of either;
• and (C) a 3′ non-translated sequence that functions to cause termination of transcription and addition of polyadenylated ribonucleotides to a 3′ end of the mRNA molecule.

A transgenic seed having a nucleic acid molecule which comprises:

• (A) an exogenous promoter region which functions to cause the production of a mRNA molecule;
• (B) a structural nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of SEQ ID NOs:
• 7, 44-77 and 50-53, complements thereof, and fragments of either;
• and (C) a 3′ non-translated sequence that functions to cause termination of transcription and addition of polyadenylated ribonucleotides to a 3′ end of the mRNA molecule.