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US 2006/0225147 A1Published application
Glyphosate-resistant plants
AssigneeMertec LLC
InventorsBruce Held, Herbert Wilson, Phllip Dykema, Carol Lewnau, Janelle Eby
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AssigneeMertec LLC
InventorsBruce Held, Herbert Wilson, Phllip Dykema, Carol Lewnau, Janelle Eby
Estimating the term…
This invention relates to glyphosate-resistant transgenic plants and methods of making the same. In a preferred embodiment, a DNA fragment which comprises an EPSPS 5′ regulatory sequence and a glyphosate-resistant EPSPS coding sequence is introduced into regenerable plant cells. The encoded EPSPS has a chloroplast transit peptide. The DNA fragment does not contain a non-EPSPS enhancer. Cells are selected for stable transformation, and the selected cells can be used to regenerate glyphosate-resistant transgenic plants. The DNA fragment used for transformation preferably comprises a modified plant genomic sequence, such as SEQ ID NO: 2, SEQ ID NO:4 or SEQ ID NO: 6. In one embodiment, two DNA fragments of this invention are stably transformed into a plant to confer glyphosate-resistance.
A method of making a glyphosate-resistant plant, comprising:
The method according to claim, further comprising introducing a second DNA fragment with the first DNA fragment into said plurality of regenerable plant cells, wherein the second DNA fragment comprises a non-heterologous EPSPS 5′ regulatory sequence and a coding sequence encoding a glyphosate-resistant EPSPS which comprises a chloroplast transit peptide.
A method of making a glyphosate-resistant plant, comprising:
Dependencies are inferred from the claim text.