Protein arrays and methods and systems for producing the same
OwnerDavid Roth, Huaping He +1inventors
InventorsDavid Roth, Huaping He, Ben Good
Life of this patent
Estimating the term…
Abstract
Methods of rapidly generating and analyzing a plurality of polypeptides are disclosed. More specifically, libraries and arrays of polypeptides are assayed in order to determine their individual immunogenic effect. Based on the immunogenic effect of polypeptides, specific subunit vaccines can be developed.
Claims18
An informatics method for ranking and then prioritizing polynucleotide sequences from a target organism for TAP amplification with subsequent protein expression and functional analyses, comprising:
- a. compiling a database of target organism polynucleotides, wherein the database includes information regarding at least one of:
- polynucleotide sequence information, amino acid sequence information, gene name, locus name, protein names, locations of open reading frames, expression of polynucleotides, expression of proteins, locations of known coding regions, and protein sizes;
- b. establishing at least one criterion for polynucleotides ranking;
- c. analyzing polynucleotides in the database for the at least one criterion;
- and
- d. ranking the polynucleotides based on the presence of criteria.
A method of identifying nucleic acids encoding immunogenic epitopes used for developing gene- and protein-based subunit vaccines, therapeutics and diagnostics comprising:
- a) analyzing a database of target organism polynucleotides for at least one criterion for ranking said polynucleotides;
- b) ranking the polynucleotides based on the presence of said criterion;
- c) preparing an array or library of polypeptides produced by the ranked or prioritized polynucleotides;
- d) screening said library to determine the ability of said polypeptides to evoke a B- or T-cell immune response.
Dependencies are inferred from the claim text.