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US 2006/0223990 A1Published application
Synthesis, deprotection, analysis & purification of RNA & ribozymes
AssigneeSirna Therapeutics, Inc.
InventorsNassim Usman, Francine Wincott, David Sweedler, Leonid Beigelman, Lech Dudycz, Susan Grimm, Anthony DiRenzo, Danuta Tracz
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Abstract
Method for purification and synthesis of RNA molecules and enzymatic RNA molecules in enzymatically active form.
Claims18
A process for chemically synthesizing, deprotecting, and purifying RNA having one or more chemical modifications, comprising:
- a. contacting a phosphoramidite with S-alkyltetrazole under conditions for synthesizing said RNA;
- b. contacting said RNA with an alkylamine under conditions for removing any exocyclic amine protecting groups or phosphate ester protecting groups;
- c. contacting said RNA with triethylamine-hydrogen fluoride under conditions to remove any alkylsilyl protecting groups from said RNA;
- d. loading said RNA onto an anion exchange high-performance liquid chromatography (HPLC) column;
- e. eluting said RNA by passing a buffer through said column;
- and
- f. collecting the eluate from said column and recovering said RNA from said eluate, under conditions which allow for the purification of said RNA.
4 more elements
Dependencies are inferred from the claim text.