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US 2006/0223986 A1Published application
Process for selectively isolating IgY antibodies from egg yolk of an anseriform bird and igy antibodies obtained thereby
AssigneeGood Biotech Corporation
InventorVictor Chiou
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Abstract
The present invention mainly relates to a process for isolation and purification of yolk antibodies from egg yolk of an anseriform bird by an adsorption chromatographic procedure using a water insoluble non-charged absorbent to accomplish a desired separation of yolk antibodies, and by a salting-out procedure that differentially precipitates the IgY antibodies. The present invention also relates to the yolk antibodies produced thereby and various uses of such yolk antibodies.
Claims21
A process for selectively isolating IgY antibodies from egg yolk of an anseriform bird, comprising:
- (a) adsorbing IgY antibodies in a water-miscible fraction obtained from the egg yolk of an anseriform bird with a porous water insoluble non-charged adsorbent selected from the group consisting of silicate, silicon compounds, carbonate, sulfate, phosphate, carbon, cellulose, synthetic fiber, ceramics, and metal oxide, and wherein the porous water insoluble non-charged adsorbent is at an amount effective for separating the IgY antibodies from the water-miscible fraction;
- and
- (b) flowing the porous water insoluble non-charged adsorbent with a buffer to obtain an aqueous fraction containing the IgY antibodies, wherein the IgY antibodies comprise antibodies with Fc regions and antibodies that lack Fc regions.
Dependencies are inferred from the claim text.