Vectors comprising guinea pig CMV regulatory elements
AssigneeMillipore Corporation
InventorsSteven Williams, Alistair Irvine, Jonathan Gawn
Life of this patent
Estimating the term…
Abstract
The invention comprises novel polynucleotides, and related vectors, host cells, methods, and compositions, containing transcriptional enhancers providing very high levels of expression of operably-linked expressible nucleic acid sequences in eukaryotic cells. Advantageously the enhancers may be used in combination with their naturally-associated promoters and/or other genetic elements that increase transcription. The invention comprises eukaryotic expression vectors that are capable of providing increased levels of expression in many cell types over that obtainable from human or murine CMV IE enhancer/promoter elements.
Claims18
An isolated polynucleotide comprising
- a. an element comprising at least 200 contiguous nucleotides of SEQ ID NO:1 and
- b. an element comprising an expressible polynucleotide sequence;
- characterised in that said isolated polynucleotide comprises, in a 5′ to 3′ direction with respect to the sense strand of the expressible polynucleotide sequence, an enhancer, a single promoter, and said expressible polynucleotide sequence, and wherein said enhancer is operably linked to said promoter, which is directly operably linked to said expressible polynucleotide sequence and wherein said promoter is not naturally operably linked to said expressible polynucleotide sequence.
Dependencies are inferred from the claim text.