Transport peptides such as C-terminal Erns peptide and analogues thereof
AssigneePepscan Systems B.V.
InventorJohannes Langedijk
Life of this patent
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Abstract
The invention relates to peptides derived from or similar to the Erns protein of pestiviruses for type-specific diagnosis of infection, for eliciting antibiotic activity, and for transport of substances into a cell. For these purposes, the invention provides, among other things, an isolated, synthetic or recombinant protein or peptide module or functional equivalent thereof comprising an amino acid sequence having significant homology to, for example, an amino acid sequence of a peptide located from about amino acid position (a) 194 to about 220 in a pestiviral Erns protein (b) 59 to about 88 in an L3 loop of a cytotoxic RNase of a ribosome-inactivating protein, or (c) 187 to about 223 in a respiratory syncytial virus G-protein.
Claims16
An isolated peptide module comprising an amino acid sequence at least 50% identical to a heparin-binding amino acid sequence.
A pharmaceutical composition comprising:
- a peptide module or equivalent of said peptide module having heparin-binding activity, said peptide module comprising an amino acid sequence selected from the group of amino acid sequences located at:
- from about amino acid position 194 to about amino acid position 220 in a pestiviral Erns protein RNase,
- from about amino acid position 59 to about amino acid position 88 in a L3 loop of a cytotoxic RNase of a ribosome-inactivating protein, or
- from about amino acid position 187 to about amino acid position 223 in a respiratory syncytial virus G-protein, and
- mixtures of any thereof;
- and
- a compound for delivery.
A method of eliciting antibiotic activity in a subject, said method comprising:
- administering, to the subject, a peptide module or equivalent of said peptide module having heparin-binding activity, said peptide module comprising an amino acid sequence selected from the group of amino acid sequences located at:
- from about amino acid position 194 to about amino acid position 220 in a pestiviral Erns protein RNase,
- from about amino acid position 59 to about amino acid position 88 in a L3 loop of a cytotoxic RNase of a ribosome-inactivating protein, or
- from about amino acid position 187 to about amino acid position 223 in a respiratory syncytial virus G-protein, and
- mixtures thereof.
A method for translocating a compound through a cell's membrane, said method comprising:
- providing said compound with an isolated peptide module or equivalent of said isolated peptide module having heparin-binding activity, said peptide module comprising an amino acid sequence selected from the group of amino acid sequences located at:
- from about amino acid position 194 to about amino acid position 220 in a pestiviral Erns protein RNase,
- from about amino acid position 59 to about amino acid position 88 in a L3 loop of a cytotoxic RNase of a ribosome-inactivating protein, or
- from about amino acid position 187 to about amino acid position 223 in a respiratory syncytial virus G-protein, and
- mixtures thereof;
- and
- contacting said compound and isolated peptide module.
Dependencies are inferred from the claim text.