Free thyroxine and free triiodothyronine analysis by mass spectrometry
AssigneeGeorgetown University
InventorSteven Soldin
Life of this patent
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Abstract
Methods, systems and kits for the simultaneous or sequential analysis of one or more hormones by mass spectrometry are disclosed. The methods require minimal sample size and minimal preparation time. The methods comprise ionizing the hormones and analyzing the hormones by mass spectrometry. In addition, methods, systems and kits for the simultaneous or sequential analysis of free thyroxine (FT4) hormone and free-triiodothyronine (FT3) is disclosed comprising ionization of the FT4 and FT3 hormone in the negative mode using an electrospray source.
Claims78
A method for mass spectrometric analysis of a sample containing or suspected of containing free thyroxine (FT4) hormone, comprising the steps:
- (a) providing a sample containing or suspected of containing FT4 hormone;
- (b) separating FT4 hormone from the sample;
- (c) collecting FT4 hormone;
- and
- (d) analyzing FT4 hormone using a mass spectrometer.
A method for mass spectrometric analysis of a sample containing or suspected of containing free triiodothyronine (FT3) hormone, comprising the steps:
- (a) providing a sample containing or suspected of containing FT3 hormone;
- (b) separating FT3 hormone from the sample;
- (c) collecting FT3 hormone;
- and
- (d) analyzing FT3 hormone using a mass spectrometer.
A method for mass spectrometric analysis of a sample containing or suspected of containing FT4 hormone and FT3 hormone, comprising the steps:
- (a) providing the sample containing or suspected of containing FT4 and FT3;
- (b) separating FT4 hormone and FT3 hormone from the sample;
- (c) collecting FT4 and FT3 hormone;
- and
- (e) analyzing FT4 and FT3 hormone using a mass spectrometer.
A system for the mass spectrometric analysis of a sample containing or suspected of containing FT4, comprising:
- (a) reagents for separating FT4 from the sample, including internal standards;
- (b) reagents for analyzing FT4 hormone using a mass spectrometer;
- and
- (c) a mass spectrometer.
A system for the mass spectrometric analysis of a sample containing or suspected of containing FT3, comprising:
- (a) reagents for separating FT3 from the sample, including internal standards;
- (b) reagents for analyzing FT3 hormone using a mass spectrometer;
- and
- (c) a mass spectrometer.
A kit for use in mass spectrometric analysis of a sample containing or suspected of containing FT4, comprising:
- (a) reagents for separating FT4 from the sample;
- (b) reagents for analyzing the FT4 using a mass spectrometer;
- (c) a solution of FT4;
- and
- (d) instructions for analyzing the FT4 using a mass spectrometer.
A kit for use in mass spectrometric analysis of a sample containing or suspected of containing FT3, comprising:
- (a) reagents for separating FT3 from the sample;
- (b) reagents for analyzing the FT3 using a mass spectrometer;
- (c) a solution of FT3;
- and
- (d) instructions for analyzing the FT3 using a mass spectrometer.
A kit for use in mass spectrometric analysis of a sample containing or suspected of containing FT3 and FT4, comprising:
- (a) reagents for separating FT3 and FT4 from the sample;
- (b) reagents for analyzing the FT3 and FT4 using a mass spectrometer;
- (c) a solution of FT3 and FT4;
- and
- (d) instructions for analyzing the FT3 and FT4 using a mass spectrometer.
Use of a mass spectrometer for analyzing a sample containing or suspected of containing FT4, FT3 or both hormones.
Dependencies are inferred from the claim text.