Preparation of platelet analogs
AssigneeBIO-RAD LABORATORIES, INC., a corporation of the state of Delaware
InventorsFranklin Carver, James Lapicola
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Abstract
Erythrocytes from a vertebrate animal other than a human that have been size reduced and treated with a fixing agent by conventional methods to resemble human platelets are further treated to achieve a size distribution that is detected in a consistent manner by different methodologies of blood cell counting. The further treatment includes heating to a denaturing temperature followed by a second treatment with a fixing agent. The resulting cells are useful as controls for calibrating and checking the accuracy of automated blood cell counting equipment.
Claims30
A process for the manufacture of human platelet analogs for use as reference controls in automated blood cell analyzers, said process comprising:
- a) treating red blood cells of a vertebrate other than a human that have been reduced in size by extraction of cellular fluid and fixed with a fixing agent, by heating said cells in the absence of a fixing agent to a sufficient degree to achieve a denaturing effect, and
- b) reacting said cells with a fixing agent after said heating.
Modified blood cells for use as analogs of human platelets in reference controls for automated blood cell analyzers, prepared by a process comprising:
- a) treating red blood cells of a vertebrate other than a human that have been reduced in size by extraction of cellular fluid and fixed with a fixing agent, by heating said cells to a denaturing temperature in the absence of a fixing agent for a sufficient period of time to achieve a denaturing effect, and
- b) reacting said cells with a fixing agent after said heating.
Dependencies are inferred from the claim text.