Supports useful in incorporating biomolecules into cells and methods of using thereof
OwnerAnthony Frutos, Joydeep Lahiri +3inventors
InventorsAnthony Frutos, Joydeep Lahiri, Santona Pal, Elizabeth Tran, Brian Webb
Life of this patent
Estimating the term…
Abstract
Described herein are supports useful in incorporating biomolecules into cells and methods of making and using thereof.
Claims126
A method for incorporating a biomolecule into a cell, comprising contacting the cell with a support comprising a substrate, a tie layer, a hydrogel layer, at least one biomolecule, and a cell, wherein the tie layer is covalently bonded to the substrate, the hydrogel layer is attached to the tie layer, the biomolecule is not covalently bonded to the hydrogel layer, and the cell is attached to the hydrogel layer.
A method for incorporating a biomolecule into a cell, comprising contacting the cell with a support comprising a substrate, a tie layer, a hydrogel layer, at least one biomolecule, and a cell, wherein the tie layer is covalently bonded to the substrate, the hydrogel layer is attached to the tie layer, the biomolecule is covalently bonded to the hydrogel layer, and the cell is attached to the hydrogel layer.
A method for detecting the activity of a biomolecule, comprising (a) contacting a support comprising a substrate, a tie layer, a hydrogel layer, the biomolecule, and cell, wherein the tie layer is covalently bonded to the substrate, the hydrogel layer is attached to the tie layer, the biomolecule is not covalently bonded to the hydrogel layer, and the cell is attached to the hydrogel layer, wherein the biomolecule is incorporated into the cell and modulates a response, and (b) detecting the response.
A support comprising a substrate, a tie layer, a hydrogel layer, at least one biomolecule, and a cell, wherein the tie layer is covalently bonded to the substrate, the hydrogel layer is attached to the tie layer, the biomolecule is not covalently bonded to the hydrogel layer, and the cell is attached to the hydrogel layer.
A support comprising a substrate, a tie layer, a hydrogel layer, at least one biomolecule, and a cell, wherein the tie layer is covalently bonded to the substrate, the hydrogel layer is attached to the tie layer, the biomolecule is covalently bonded to the hydrogel layer, and the cell is attached to the hydrogel layer.
Dependencies are inferred from the claim text.